An autophagy assay reveals the ESCRT-III component CHMP2A as a regulator of phagophore closure

Yoshinori Takahashi; Haiyan He; Zhenyuan Tang; Tatsuya Hattori; Ying Liu; Megan M. Young; Jacob M. Serfass; Longgui Chen; Melat Gebru; Chong Chen; Carson A. Wills; Jennifer M. Atkinson; Han Chen; Thomas Abraham; Hong-Gang Wang

doi:10.1038/s41467-018-05254-w

Nature Communications (Jul 2018)

An autophagy assay reveals the ESCRT-III component CHMP2A as a regulator of phagophore closure

Yoshinori Takahashi,
Haiyan He,
Zhenyuan Tang,
Tatsuya Hattori,
Ying Liu,
Megan M. Young,
Jacob M. Serfass,
Longgui Chen,
Melat Gebru,
Chong Chen,
Carson A. Wills,
Jennifer M. Atkinson,
Han Chen,
Thomas Abraham,
Hong-Gang Wang

Affiliations

Yoshinori Takahashi: Department of Pediatrics, Penn State College of Medicine
Haiyan He: Department of Pediatrics, Penn State College of Medicine
Zhenyuan Tang: Department of Pediatrics, Penn State College of Medicine
Tatsuya Hattori: Department of Pediatrics, Penn State College of Medicine
Ying Liu: Department of Pediatrics, Penn State College of Medicine
Megan M. Young: Department of Pediatrics, Penn State College of Medicine
Jacob M. Serfass: Department of Pharmacology, Penn State College of Medicine
Longgui Chen: Department of Pediatrics, Penn State College of Medicine
Melat Gebru: Department of Pediatrics, Penn State College of Medicine
Chong Chen: Department of Pediatrics, Penn State College of Medicine
Carson A. Wills: Department of Pediatrics, Penn State College of Medicine
Jennifer M. Atkinson: Department of Pediatrics, Penn State College of Medicine
Han Chen: Microscopy Imaging Facility, Penn State College of Medicine
Thomas Abraham: Microscopy Imaging Facility, Penn State College of Medicine
Hong-Gang Wang: Department of Pediatrics, Penn State College of Medicine

DOI: https://doi.org/10.1038/s41467-018-05254-w
Journal volume & issue: Vol. 9, no. 1
pp. 1 – 13

Abstract

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During autophagy, phagophores elongate to form double-membrane vesicles but the mechanism behind their closure is unknown. Here, the authors develop an autophagy assay and find a role for the endosomal sorting complexes required for transport component CHMP2A as a phagophore closure regulator.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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