Nature Communications (Feb 2020)

Benchmarking tomographic acquisition schemes for high-resolution structural biology

  • Beata Turoňová,
  • Wim J. H. Hagen,
  • Martin Obr,
  • Shyamal Mosalaganti,
  • J. Wouter Beugelink,
  • Christian E. Zimmerli,
  • Hans-Georg Kräusslich,
  • Martin Beck

DOI
https://doi.org/10.1038/s41467-020-14535-2
Journal volume & issue
Vol. 11, no. 1
pp. 1 – 9

Abstract

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Here the authors systematically benchmark cryo-electron tomography acquisition schemes to optimize the attainable resolution for subtomogram averaging, and find that dose-symmetric acquisition with even angular sampling provides a better outcome than most currently used acquisition schemes.