Protective Effects of Baccharis dracunculifolia Leaves Extract against Carbon Tetrachloride- and Acetaminophen-Induced Hepatotoxicity in Experimental Animals
Túlio P. Rezende,
José Otávio do A. Corrêa,
Beatriz J. V. Aarestrup,
Fernando M. Aarestrup,
Orlando V. de Sousa,
Ademar A. da Silva Filho
Affiliations
Túlio P. Rezende
Identificação e Pesquisa em Princípios Ativos Naturais - NIPPAN, Faculdade de Farmácia, Universidade Federal de Juiz de Fora, Rua José Lourenço Kelmer, s/n – Campus Universitário, Bairro São Pedro, CEP36036-900, Juiz de Fora, MG, Brazil
José Otávio do A. Corrêa
Laboratório de Bioatividade Celular e Molecular, Universidade Federal de Juiz de Fora, Rua José Lourenço Kelmer, s/n – Campus Universitário, Bairro São Pedro, CEP36036-900, Juiz de Fora, MG, Brazil
Beatriz J. V. Aarestrup
Laboratório de Imunopatologia e Patologia Experimental, Universidade Federal de Juiz de Fora, Rua José Lourenço Kelmer, s/n – Campus Universitário, Bairro São Pedro, CEP36036-900, Juiz de Fora, MG, Brazil
Fernando M. Aarestrup
Laboratório de Imunopatologia e Patologia Experimental, Universidade Federal de Juiz de Fora, Rua José Lourenço Kelmer, s/n – Campus Universitário, Bairro São Pedro, CEP36036-900, Juiz de Fora, MG, Brazil
Orlando V. de Sousa
Laboratório de Farmacologia de Produtos Naturais, Universidade Federal de Juiz de Fora, Rua José Lourenço Kelmer, s/n – Campus Universitário, Bairro São Pedro, CEP36036-900, Juiz de Fora, MG, Brazil
Ademar A. da Silva Filho
Identificação e Pesquisa em Princípios Ativos Naturais - NIPPAN, Faculdade de Farmácia, Universidade Federal de Juiz de Fora, Rua José Lourenço Kelmer, s/n – Campus Universitário, Bairro São Pedro, CEP36036-900, Juiz de Fora, MG, Brazil
In this work we investigated the in vivo protective effects of Baccharis dracunculifolia leaves extract (BdE) against carbon tetrachloride (CCl4)- and acetaminophen (APAP)-induced hepatotoxicity. Total phenolic content, total flavonoid content, antioxidant DPPH radical scavenging activity, and HPLC analysis were performed. Our results showed that pretreatment with BdE significantly reduced the damage caused by CCl4 and APAP on the serum markers of hepatic injury, AST, ALT, and ALP. Results were confirmed by histopathological analysis. Phytochemical analysis, performed by HPLC, showed that BdE was rich in p-coumaric acid derivatives, caffeoylquinic acids and flavonoids. BdE also showed DPPH antioxidant activity (EC50 of 15.75 ± 0.43 μg/mL), and high total phenolic (142.90 ± 0.77 mg GAE/g) and flavonoid (51.47 ± 0.60 mg RE/g) contents. This study indicated that B. dracunculifolia leaves extract has relevant in vivo hepatoprotective properties.
