mBio
(Feb 2021)
Metagenomic Quantification of Genes with Internal Standards
Emily Crossette,
Jordan Gumm,
Kathryn Langenfeld,
Lutgarde Raskin,
Melissa Duhaime,
Krista Wigginton
Affiliations
Emily Crossette
Department of Civil and Environmental Engineering, University of Michigan, Ann Arbor, Michigan, USA
Jordan Gumm
Ecology and Evolutionary Biology, University of Michigan, Ann Arbor, Michigan, USA
Kathryn Langenfeld
Department of Civil and Environmental Engineering, University of Michigan, Ann Arbor, Michigan, USA
Lutgarde Raskin
Department of Civil and Environmental Engineering, University of Michigan, Ann Arbor, Michigan, USA
Melissa Duhaime
ORCiD Ecology and Evolutionary Biology, University of Michigan, Ann Arbor, Michigan, USA
Krista Wigginton
ORCiD Department of Civil and Environmental Engineering, University of Michigan, Ann Arbor, Michigan, USA
DOI
https://doi.org/10.1128/mBio.03173-20
Journal volume & issue
Vol. 12,
no. 1
Abstract
Read online
qPCR and metagenomics are central molecular techniques that have offered insights into biological processes for decades, from monitoring spatial and temporal gene dynamics to tracking ARGs or pathogens. Still needed is a tool that can quantify thousands of relevant genes in a sample as gene copies per sample mass or volume.
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