Frontiers in Endocrinology (Jun 2023)
Evidence of causality of low body mass index on risk of adolescent idiopathic scoliosis: a Mendelian randomization study
- Nao Otomo,
- Nao Otomo,
- Nao Otomo,
- Anas M. Khanshour,
- Masaru Koido,
- Masaru Koido,
- Kazuki Takeda,
- Yukihide Momozawa,
- Michiaki Kubo,
- Yoichiro Kamatani,
- Yoichiro Kamatani,
- John A. Herring,
- John A. Herring,
- Yoji Ogura,
- Yohei Takahashi,
- Shohei Minami,
- Koki Uno,
- Noriaki Kawakami,
- Manabu Ito,
- Tatsuya Sato,
- Kei Watanabe,
- Takashi Kaito,
- Haruhisa Yanagida,
- Hiroshi Taneichi,
- Katsumi Harimaya,
- Yuki Taniguchi,
- Hideki Shigematsu,
- Takahiro Iida,
- Takahiro Iida,
- Satoru Demura,
- Ryo Sugawara,
- Nobuyuki Fujita,
- Nobuyuki Fujita,
- Mitsuru Yagi,
- Mitsuru Yagi,
- Eijiro Okada,
- Naobumi Hosogane,
- Naobumi Hosogane,
- Katsuki Kono,
- Katsuki Kono,
- Masaya Nakamura,
- Kazuhiro Chiba,
- Kazuhiro Chiba,
- Toshiaki Kotani,
- Tsuyoshi Sakuma,
- Tsutomu Akazawa,
- Teppei Suzuki,
- Kotaro Nishida,
- Kenichiro Kakutani,
- Taichi Tsuji,
- Hideki Sudo,
- Akira Iwata,
- Satoshi Inami,
- Carol A. Wise,
- Carol A. Wise,
- Carol A. Wise,
- Yuta Kochi,
- Morio Matsumoto,
- Shiro Ikegawa,
- Kota Watanabe,
- Chikashi Terao,
- Chikashi Terao,
- Chikashi Terao
Affiliations
- Nao Otomo
- Laboratory for Statistical and Translational Genetics, RIKEN Center for Integrative Medical Sciences, RIKEN, Yokohama, Japan
- Nao Otomo
- Department of Orthopaedic Surgery, Keio University School of Medicine, Tokyo, Japan
- Nao Otomo
- Laboratory for Bone and Joint Diseases, RIKEN Center for Integrative Medical Sciences, Tokyo, Japan
- Anas M. Khanshour
- Center for Translational Research, Scottish Rite for Children, Dallas, TX, United States
- Masaru Koido
- Laboratory for Statistical and Translational Genetics, RIKEN Center for Integrative Medical Sciences, RIKEN, Yokohama, Japan
- Masaru Koido
- Division of Molecular Pathology, Institute of Medical Science, The University of Tokyo, Tokyo, Japan
- Kazuki Takeda
- Department of Orthopaedic Surgery, Keio University School of Medicine, Tokyo, Japan
- Yukihide Momozawa
- Laboratory for Genotyping Development, RIKEN Center for Integrative Medical Sciences, Yokohama, Japan
- Michiaki Kubo
- Laboratory for Genotyping Development, RIKEN Center for Integrative Medical Sciences, Yokohama, Japan
- Yoichiro Kamatani
- Division of Molecular Pathology, Institute of Medical Science, The University of Tokyo, Tokyo, Japan
- Yoichiro Kamatani
- Laboratory of Complex Trait Genomics, Graduate School of Frontier Science, The University of Tokyo, Tokyo, Japan
- John A. Herring
- Department of Orthopaedic Surgery , Scottish Rite for Children, Dallas, TX, United States
- John A. Herring
- Department of Orthopaedic Surgery and Pediatric, University of Texas Southwestern Medical Center, Dallas, TX, United States
- Yoji Ogura
- Department of Orthopaedic Surgery, Keio University School of Medicine, Tokyo, Japan
- Yohei Takahashi
- Department of Orthopaedic Surgery, Keio University School of Medicine, Tokyo, Japan
- Shohei Minami
- 0Department of Orthopaedic Surgery, Seirei Sakura Citizen Hospital, Sakura, Japan
- Koki Uno
- 1Department of Orthopaedic Surgery, National Hospital Organization, Kobe Medical Center, Kobe, Japan
- Noriaki Kawakami
- 2Department of Orthopaedic Surgery, Meijo Hospital, Nagoya, Japan
- Manabu Ito
- 3Department of Orthopaedic Surgery, National Hospital Organization, Hokkaido Medical Center, Sapporo, Japan
- Tatsuya Sato
- 4Department of Orthopaedic Surgery, Juntendo University School of Medicine, Tokyo, Japan
- Kei Watanabe
- 5Department of Orthopaedic Surgery, Niigata University Medical and Dental General Hospital, Niigata, Japan
- Takashi Kaito
- 6Department of Orthopaedic Surgery, Osaka University Graduate School of Medicine, Suita, Japan
- Haruhisa Yanagida
- 7Department of Orthopaedic and Spine Surgery, Fukuoka Children’s Hospital, Fukuoka, Japan
- Hiroshi Taneichi
- 8Department of Orthopaedic Surgery, Dokkyo Medical University School of Medicine, Mibu, Japan
- Katsumi Harimaya
- 9Department of Orthopaedic Surgery, Kyushu University Beppu Hospital, Beppu, Japan
- Yuki Taniguchi
- 0Department of Orthopaedic Surgery, Faculty of Medicine, The University of Tokyo, Tokyo, Japan
- Hideki Shigematsu
- 1Department of Orthopaedic Surgery, Nara Medical University, Kashihara, Japan
- Takahiro Iida
- 2Department of Orthopaedic Surgery, Dokkyo Medical University Saitama Medical Center, Koshigaya, Japan
- Takahiro Iida
- 3Department of Orthopaedic Surgery, Teine Keijinkai Hospital, Sapporo, Japan
- Satoru Demura
- 4Department of Orthopaedic Surgery Graduated School of Medical Science, Kanazawa University, Kanazawa, Japan
- Ryo Sugawara
- 5Department of Orthopaedic Surgery, Jichi Medical University, Shimotsuke, Japan
- Nobuyuki Fujita
- Department of Orthopaedic Surgery, Keio University School of Medicine, Tokyo, Japan
- Nobuyuki Fujita
- 6Department of Orthopaedic Surgery, Fujita Health University, Toyoake, Japan
- Mitsuru Yagi
- Department of Orthopaedic Surgery, Keio University School of Medicine, Tokyo, Japan
- Mitsuru Yagi
- 7Department of Orthopaedic Surgery, International University of Health and Welfare School of Medicine, Narita, Japan
- Eijiro Okada
- Department of Orthopaedic Surgery, Keio University School of Medicine, Tokyo, Japan
- Naobumi Hosogane
- Department of Orthopaedic Surgery, Keio University School of Medicine, Tokyo, Japan
- Naobumi Hosogane
- 8Department of Orthopaedic Surgery, National Defense Medical College, Tokorozawa, Japan
- Katsuki Kono
- Department of Orthopaedic Surgery, Keio University School of Medicine, Tokyo, Japan
- Katsuki Kono
- 9Department of Orthopaedic Surgery, Kono Orthopaedic Clinic, Tokyo, Japan
- Masaya Nakamura
- Department of Orthopaedic Surgery, Keio University School of Medicine, Tokyo, Japan
- Kazuhiro Chiba
- Department of Orthopaedic Surgery, Keio University School of Medicine, Tokyo, Japan
- Kazuhiro Chiba
- 6Department of Orthopaedic Surgery, Fujita Health University, Toyoake, Japan
- Toshiaki Kotani
- 0Department of Orthopaedic Surgery, Seirei Sakura Citizen Hospital, Sakura, Japan
- Tsuyoshi Sakuma
- 0Department of Orthopaedic Surgery, Seirei Sakura Citizen Hospital, Sakura, Japan
- Tsutomu Akazawa
- 0Department of Orthopaedic Surgery, Seirei Sakura Citizen Hospital, Sakura, Japan
- Teppei Suzuki
- 1Department of Orthopaedic Surgery, National Hospital Organization, Kobe Medical Center, Kobe, Japan
- Kotaro Nishida
- 0Department of Orthopaedic Surgery, Kobe University Graduate School of Medicine, Kobe, Japan
- Kenichiro Kakutani
- 0Department of Orthopaedic Surgery, Kobe University Graduate School of Medicine, Kobe, Japan
- Taichi Tsuji
- 2Department of Orthopaedic Surgery, Meijo Hospital, Nagoya, Japan
- Hideki Sudo
- 1Department of Advanced Medicine for Spine and Spinal Cord Disorders, Hokkaido University Graduate School of Medicine, Sapporo, Japan
- Akira Iwata
- 2Department of Preventive and Therapeutic Research for Metastatic Bone Tumor, Faculty of Medicine and Graduate School of Medicine, Hokkaido University, Sapporo, Japan
- Satoshi Inami
- 8Department of Orthopaedic Surgery, Dokkyo Medical University School of Medicine, Mibu, Japan
- Carol A. Wise
- Center for Translational Research, Scottish Rite for Children, Dallas, TX, United States
- Carol A. Wise
- Department of Orthopaedic Surgery and Pediatric, University of Texas Southwestern Medical Center, Dallas, TX, United States
- Carol A. Wise
- 3McDermott Center for Human Growth and Development, University of Texas Southwestern Medical Center, Dallas, TX, United States
- Yuta Kochi
- 4Department of Genomic Function and Diversity, Medical Research Institute, Tokyo Medical and Dental and University, Tokyo, Japan
- Morio Matsumoto
- Department of Orthopaedic Surgery, Keio University School of Medicine, Tokyo, Japan
- Shiro Ikegawa
- Laboratory for Bone and Joint Diseases, RIKEN Center for Integrative Medical Sciences, Tokyo, Japan
- Kota Watanabe
- Department of Orthopaedic Surgery, Keio University School of Medicine, Tokyo, Japan
- Chikashi Terao
- Laboratory for Statistical and Translational Genetics, RIKEN Center for Integrative Medical Sciences, RIKEN, Yokohama, Japan
- Chikashi Terao
- 5Clinical Research Center, Shizuoka General Hospital, Shizuoka, Japan
- Chikashi Terao
- 6Department of Applied Genetics, The School of Pharmaceutical Sciences, University of Shizuoka, Shizuoka, Japan
- DOI
- https://doi.org/10.3389/fendo.2023.1089414
- Journal volume & issue
-
Vol. 14
Abstract
IntroductionAdolescent idiopathic scoliosis (AIS) is a disorder with a three-dimensional spinal deformity and is a common disease affecting 1-5% of adolescents. AIS is also known as a complex disease involved in environmental and genetic factors. A relation between AIS and body mass index (BMI) has been epidemiologically and genetically suggested. However, the causal relationship between AIS and BMI remains to be elucidated.Material and methodsMendelian randomization (MR) analysis was performed using summary statistics from genome-wide association studies (GWASs) of AIS (Japanese cohort, 5,327 cases, 73,884 controls; US cohort: 1,468 cases, 20,158 controls) and BMI (Biobank Japan: 173430 individual; meta-analysis of genetic investigation of anthropometric traits and UK Biobank: 806334 individuals; European Children cohort: 39620 individuals; Population Architecture using Genomics and Epidemiology: 49335 individuals). In MR analyses evaluating the effect of BMI on AIS, the association between BMI and AIS summary statistics was evaluated using the inverse-variance weighted (IVW) method, weighted median method, and Egger regression (MR-Egger) methods in Japanese.ResultsSignificant causality of genetically decreased BMI on risk of AIS was estimated: IVW method (Estimate (beta) [SE] = -0.56 [0.16], p = 1.8 × 10-3), weighted median method (beta = -0.56 [0.18], p = 8.5 × 10-3) and MR-Egger method (beta = -1.50 [0.43], p = 4.7 × 10-3), respectively. Consistent results were also observed when using the US AIS summary statistic in three MR methods; however, no significant causality was observed when evaluating the effect of AIS on BMI.ConclusionsOur Mendelian randomization analysis using large studies of AIS and GWAS for BMI summary statistics revealed that genetic variants contributing to low BMI have a causal effect on the onset of AIS. This result was consistent with those of epidemiological studies and would contribute to the early detection of AIS.
Keywords
- adolescent idiopathic scoliosis
- body mass index
- Mendelian randomization (MR)
- genetic study
- genome-wide association study