Biofilm forms in mono- and mixed staphylococci species culture in combination with lactobacilli

Abstract

Insufficient effectiveness of antimicrobial treatment of many infectious inflammation processes are often associated with the formation of the microbial Biofilms forms. The ability to a biofilm formation was investigated in clinical S. aureus and S. epidermidis strains isolated from patients with acne vulgaris and reference L. рlantarum 8P-A3 strain. For evaluation of the film formation the spectrophotometric measuring of the optical density of structures at the bottom of plastic Petri dishes was used. Item condition of the film structures was also controlled by using microscopic method. It was established that the optical densities of monospecific biofilms formed by S. aureus and S. epidermidis were 1.69±0.77 and 0.60±1.50 OD, respectively, and the planktonic forms of the reference strain was 0.09±0.06 OD. Biofilm structure of L. plantarum 8P-A3 has optical density of 1.79±1.07 OD. Fluorescent microscopy allowed to estimate the proportion of the viable cells in the biofilm. The viable cells were absent after 48 h of mixed-species formation of L. рlantarum 8P-A3 and S. aureus biofilm. Local application of the preparations from lactobacilli contributes to the effectiveness of treatment of the inflammation caused by staphylococcus biofilm forms.

Keywords

• biofilm
• Lactobacillus
• Staphylococcus aureus
• Staphylococcus epidermіdis