Jichu yixue yu linchuang (Dec 2021)
Curcumin enhances the radio-sensitivity of prostate cancer cell line PC-3
Abstract
Objective To explore the molecular mechanism of curcumin increasing the radiosensitivity of prostate cancer PC-3 cells. Methods Prostate cancer PC-3 cells were cultured and treated with curcumin, irradiation or combined therapeutic methods to observe the proliferation, apoptosis and change of cell cycle of PC-3 cells shown by MTT,RT-qPCR and FCM. Results Compared with control group, the cells from the radiation or curcumin group, the survival rate, the level of PCNA mRNA and Cdc2 mRNA were decreased (P＜0.05), and the ratio of S phase and G2/M phase, caspase-3 and caspase-9 activity and the apoptosis rate were increased (P＜0.05); Compared with the cells in the radiation group, after treated with curcumin combined with radiotherapy, the survival rate, the level of PCNA mRNA and Cdc2 mRNA were decreased (P＜0.05), and the ratio of S phase and G2/M phase, caspase-3 and caspase-9 activity and the apoptosis rate were all increased (P＜0.05). Conclusions Curcumin has a certain radiosensitizing effect in vitro, which potentially strengthen the inhibitory effect of radiotherapy on the growth of prostate cancer PC-3 cells.
