DDAO Controlled Synthesis of Organo-Modified Silica Nanoparticles with Encapsulated Fluorescent Boron Dipyrrins and Study of Their Uptake by Cancerous Cells
Aleksandr A. Goncharenko,
Ilya A. Tarasyuk,
Yuriy S. Marfin,
Kirill V. Grzhegorzhevskii,
Albert R. Muslimov,
Andrey B. Bondarenko,
Maxim D. Lebedev,
Ilya A. Kuz’min,
Artur S. Vashurin,
Kirill V. Lepik,
Alexander S. Timin,
Evgeniy V. Rumyantsev
Affiliations
Aleksandr A. Goncharenko
Department of Biophysics, Polytechnicheskaya, Peter The Great St. Petersburg Polytechnic University, 29, 195251 St. Petersburg, Russia
Ilya A. Tarasyuk
Ivanovo State University of Chemistry and Technology, Sheremetevsky str., 7, 153000 Ivanovo, Russia
Yuriy S. Marfin
Ivanovo State University of Chemistry and Technology, Sheremetevsky str., 7, 153000 Ivanovo, Russia
Kirill V. Grzhegorzhevskii
Institute of Natural Sciences and Mathematics, Ural Federal University, 19, 620002 Mira St. Ekaterinburg, Russia
Albert R. Muslimov
RM Gorbacheva Research Institute of Pediatric Oncology, Hematology and Transplantation, Pavlov University, Lva Tolstogo 6/8, 197022 St. Petersburg, Russia
Andrey B. Bondarenko
St. Petersburg State University, 7/9 Universitetskaya nab., 199034 Saint-Petersburg, Russia
Maxim D. Lebedev
Ivanovo State University of Chemistry and Technology, Sheremetevsky str., 7, 153000 Ivanovo, Russia
Ilya A. Kuz’min
Ivanovo State University of Chemistry and Technology, Sheremetevsky str., 7, 153000 Ivanovo, Russia
Artur S. Vashurin
Ivanovo State University of Chemistry and Technology, Sheremetevsky str., 7, 153000 Ivanovo, Russia
Kirill V. Lepik
RM Gorbacheva Research Institute of Pediatric Oncology, Hematology and Transplantation, Pavlov University, Lva Tolstogo 6/8, 197022 St. Petersburg, Russia
Alexander S. Timin
Department of Biophysics, Polytechnicheskaya, Peter The Great St. Petersburg Polytechnic University, 29, 195251 St. Petersburg, Russia
Evgeniy V. Rumyantsev
Ivanovo State University of Chemistry and Technology, Sheremetevsky str., 7, 153000 Ivanovo, Russia
The design of cargo carriers with high biocompatibility, unique morphological characteristics, and capability of strong bonding of fluorescent dye is highly important for the development of a platform for smart imaging and diagnostics. In this paper, BODIPY-doped silica nanoparticles were prepared through a “one-pot” soft-template method using a sol-gel process. Several sol-gel precursors have been used in sol-gel synthesis in the presence of soft-template to obtain the silica-based materials with the most appropriate morphological features for the immobilization of BODIPY molecules. Obtained silica particles have been shown to be non-cytotoxic and can be effectively internalized into the cervical cancer cell line (HeLa). The described method of synthesis allows us to obtain silica-based carriers with an immobilized fluorescent dye that provide the possibility for real-time imaging and detection of these carriers.
