A standardised low-cost membrane blood-feeder for Aedes aegypti made using common laboratory materials

Abstract

Blood feeding is a necessary part of laboratory studies involving mosquitoes and other hematophagous arthropods of interest in medical and ecological research. However, methods involving hosts may present serious risks, require ethics approvals and can be expensive. Here we describe an insect blood feeder made using common laboratory materials, which is low cost (<US$100) and can be constructed and operated with little technical expertise. We compared the blood feeder containing an artificial blood diet, Skitosnack, to direct human arm feeding for Aedes aegypti (Diptera: Culicidae), in terms of engorgement rate, fecundity and hatch rate. No significant difference in fecundity between the two approaches was found, (mean ± SD); direct human arm: 56 ± 26 eggs/female, artificial method: 47 ± 25 eggs/female, P = 0.569. Engorgement rates (direct human arm: 97.8 ± 4%, artificial: 64.1 ± 23%, P < 0.05) and hatch rates (direct human arm: 75 ± 12%, artificial: 59 ± 14%, P < 0.05) were lower in the artificially fed mosquitoes. Despite these differences, we maintained a healthy mosquito colony for 10 generations using the artificial feeding approach. Results from this comparison are within the range of other studies which compared direct host feeding with an artificial feeding method. We anticipate that the blood feeder presented here could substantially reduce costs usually required to establish a standardised and effective blood feeding method for maintaining mosquito colonies or conducting experiments, extending the capability of laboratories especially where research resources are limited, but vector-borne diseases common.

Keywords

• Aedes aegypti
• Membrane feeding
• Bloodfeeding
• Artificial blood feeding