Genetic diversity of Brassica rapa germplasm of Azad Jammu and Kashmir, Pakistan revealed by molecular markers

Abstract

Brassica species commonly known as rapeseed are the second largest oil yielding crop of Pakistan and belongs to the family Brassicaceae. This scientific investigation was carried out to explore the genetic diversity of Brassica rapa from Azad Jammu and Kashmir (AJK) Pakistan. Seeds of Brassica rapa L. genotypes of Azad Jammu and Kashmir were collected from Plant Genetic Research Institute (PGRI), National Agricultural Research Centre (NARC), Islamabad Pakistan. CTAB method was used for the extraction of DNA from leaf samples. SSR and ISSR markers were used to explore the genetic diversity and cluster analysis was used for data analysis. Out of 6 SSR markers, Ra2E11 and Na10-E02 showed maximum genetic diversity while Ra2-E07, Ra2-D04, and Na10-D03 showed moderate diversity while Ra2-E01 showed minimum genetic diversity. Among the elected genotypes 026503 (Bhimber), 026514 (Kotli), 026510 (Kotli) and 026539 (Bagh) showed maximum diversity with 6 SSR markers. From 10 ISSR markers ISSR 888 and ISSR 5 showed highest number of bands (7). ISSR 3, ISSR 5, ISSR 888 showed maximum genetic diversity among mustard genotypes, while ISSR 887 and ISSR 840 showed no results and ISSR 2, ISSR 4, ISSR 80, ISSR 811, ISSR 889 showed moderate diversity. Among the selected genotypes 026557 showed maximum diversity with ISSR markers. Based upon the present studies the genotypes 026503, 026514, 026510, 026539 and 026557 had been recognised as probable most significant genotypes which could be used in future plant breeding program.

Keywords

• Brassica rapa
• Azad Kashmir
• genetic diversty
• SSR
• ISSR
• markers