A system for bioelectronic delivery of treatment directed toward wound healing

Prabhat Baniya; Maryam Tebyani; Narges Asefifeyzabadi; Tiffany Nguyen; Cristian Hernandez; Kan Zhu; Houpu Li; John Selberg; Hao-Chieh Hsieh; Pattawong Pansodtee; Hsin-ya Yang; Cynthia Recendez; Gordon Keller; Wan Shen Hee; Elham Aslankoohi; Roslyn Rivkah Isseroff; Min Zhao; Marcella Gomez; Marco Rolandi; Mircea Teodorescu

doi:10.1038/s41598-023-41572-w

Scientific Reports (Sep 2023)

A system for bioelectronic delivery of treatment directed toward wound healing

Prabhat Baniya,
Maryam Tebyani,
Narges Asefifeyzabadi,
Tiffany Nguyen,
Cristian Hernandez,
Kan Zhu,
Houpu Li,
John Selberg,
Hao-Chieh Hsieh,
Pattawong Pansodtee,
Hsin-ya Yang,
Cynthia Recendez,
Gordon Keller,
Wan Shen Hee,
Elham Aslankoohi,
Roslyn Rivkah Isseroff,
Min Zhao,
Marcella Gomez,
Marco Rolandi,
Mircea Teodorescu

Affiliations

Prabhat Baniya: Department of Electrical and Computer Engineering, University of California Santa Cruz
Maryam Tebyani: Department of Electrical and Computer Engineering, University of California Santa Cruz
Narges Asefifeyzabadi: Department of Electrical and Computer Engineering, University of California Santa Cruz
Tiffany Nguyen: Department of Electrical and Computer Engineering, University of California Santa Cruz
Cristian Hernandez: Department of Electrical and Computer Engineering, University of California Santa Cruz
Kan Zhu: Department of Dermatology, School of Medicine, University of California Davis
Houpu Li: Department of Electrical and Computer Engineering, University of California Santa Cruz
John Selberg: Department of Electrical and Computer Engineering, University of California Santa Cruz
Hao-Chieh Hsieh: Department of Electrical and Computer Engineering, University of California Santa Cruz
Pattawong Pansodtee: Department of Electrical and Computer Engineering, University of California Santa Cruz
Hsin-ya Yang: Department of Dermatology, School of Medicine, University of California Davis
Cynthia Recendez: Department of Dermatology, School of Medicine, University of California Davis
Gordon Keller: Department of Electrical and Computer Engineering, University of California Santa Cruz
Wan Shen Hee: Department of Electrical and Computer Engineering, University of California Santa Cruz
Elham Aslankoohi: Department of Electrical and Computer Engineering, University of California Santa Cruz
Roslyn Rivkah Isseroff: Department of Dermatology, School of Medicine, University of California Davis
Min Zhao: Department of Dermatology, School of Medicine, University of California Davis
Marcella Gomez: Department of Applied Mathematics, University of California Santa Cruz
Marco Rolandi: Department of Electrical and Computer Engineering, University of California Santa Cruz
Mircea Teodorescu: Department of Electrical and Computer Engineering, University of California Santa Cruz

DOI: https://doi.org/10.1038/s41598-023-41572-w
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 17

Abstract

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Abstract The development of wearable bioelectronic systems is a promising approach for optimal delivery of therapeutic treatments. These systems can provide continuous delivery of ions, charged biomolecules, and an electric field for various medical applications. However, rapid prototyping of wearable bioelectronic systems for controlled delivery of specific treatments with a scalable fabrication process is challenging. We present a wearable bioelectronic system comprised of a polydimethylsiloxane (PDMS) device cast in customizable 3D printed molds and a printed circuit board (PCB), which employs commercially available engineering components and tools throughout design and fabrication. The system, featuring solution-filled reservoirs, embedded electrodes, and hydrogel-filled capillary tubing, is assembled modularly. The PDMS and PCB both contain matching through-holes designed to hold metallic contact posts coated with silver epoxy, allowing for mechanical and electrical integration. This assembly scheme allows us to interchange subsystem components, such as various PCB designs and reservoir solutions. We present three PCB designs: a wired version and two battery-powered versions with and without onboard memory. The wired design uses an external voltage controller for device actuation. The battery-powered PCB design uses a microcontroller unit to enable pre-programmed applied voltages and deep sleep mode to prolong battery run time. Finally, the battery-powered PCB with onboard memory is developed to record delivered currents, which enables us to verify treatment dose delivered. To demonstrate the functionality of the platform, the devices are used to deliver H $$^+$$ + in vivo using mouse models and fluoxetine ex vivo using a simulated wound environment. Immunohistochemistry staining shows an improvement of 35.86% in the M1/M2 ratio of H $$^+$$ + —treated wounds compared with control wounds, indicating the potential of the platform to improve wound healing.

Published in Scientific Reports

ISSN: 2045-2322 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Medicine; Science
Website: https://www.nature.com/srep/

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