Frontiers in Oncology (Jun 2021)

A Non-Coding RNA Network Involved in KSHV Tumorigenesis

  • Julián Naipauer,
  • Julián Naipauer,
  • Martín E. García Solá,
  • Martín E. García Solá,
  • Martín E. García Solá,
  • Daria Salyakina,
  • Santas Rosario,
  • Sion Williams,
  • Sion Williams,
  • Omar Coso,
  • Omar Coso,
  • Omar Coso,
  • Martín C. Abba,
  • Martín C. Abba,
  • Enrique A. Mesri,
  • Enrique A. Mesri,
  • Ezequiel Lacunza,
  • Ezequiel Lacunza

DOI
https://doi.org/10.3389/fonc.2021.687629
Journal volume & issue
Vol. 11

Abstract

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Regulatory pathways involving non-coding RNAs (ncRNAs), such as microRNAs (miRNAs) and long non-coding RNAs (lncRNA), have gained great relevance due to their role in the control of gene expression modulation. Using RNA sequencing of KSHV Bac36 transfected mouse endothelial cells (mECK36) and tumors, we have analyzed the host and viral transcriptome to uncover the role lncRNA-miRNA-mRNA driven networks in KSHV tumorigenesis. The integration of the differentially expressed ncRNAs, with an exhaustive computational analysis of their experimentally supported targets, led us to dissect complex networks integrated by the cancer-related lncRNAs Malat1, Neat1, H19, Meg3, and their associated miRNA-target pairs. These networks would modulate pathways related to KSHV pathogenesis, such as viral carcinogenesis, p53 signaling, RNA surveillance, and cell cycle control. Finally, the ncRNA-mRNA analysis allowed us to develop signatures that can be used to an appropriate identification of druggable gene or networks defining relevant AIDS-KS therapeutic targets.

Keywords