Nature Communications (Nov 2021)

BEAR reveals that increased fidelity variants can successfully reduce the mismatch tolerance of adenine but not cytosine base editors

  • András Tálas,
  • Dorottya A. Simon,
  • Péter I. Kulcsár,
  • Éva Varga,
  • Sarah L. Krausz,
  • Ervin Welker

DOI
https://doi.org/10.1038/s41467-021-26461-y
Journal volume & issue
Vol. 12, no. 1
pp. 1 – 14

Abstract

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Base editors allow for precision engineering of the genome. Here, the authors present BEAR, a plasmid-based fluorescence assay for the measurement of CBE and ABE activity, to reveal the mechanism underlying their differences and to increase the yield of edited cells with reduced indel background.