Novel <i>Chaphamaparvovirus</i> in Insectivorous <i>Molossus molossus</i> Bats, from the Brazilian Amazon Region
Endrya do Socorro Foro Ramos,
Wandercleyson Uchôa Abreu,
Luis Reginaldo Ribeiro Rodrigues,
Luis Fernando Marinho,
Vanessa dos Santos Morais,
Fabiola Villanova,
Ramendra Pati Pandey,
Emerson Luiz Lima Araújo,
Xutao Deng,
Eric Delwart,
Antonio Charlys da Costa,
Elcio Leal
Affiliations
Endrya do Socorro Foro Ramos
Laboratório de Diversidade Viral, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belem 66075-000, Pará, Brazil
Wandercleyson Uchôa Abreu
Programa de Pos-Graduação REDE Bionorte, Polo Pará, Universidade Federal do Oeste do Pará, Santarém 68040-255, Pará, Brazil
Luis Reginaldo Ribeiro Rodrigues
Laboratory of Genetics & Biodiversity, Institute of Educational Sciences, Universidade Federal do Oeste do Pará, Santarém 68040-255, Pará, Brazil
Luis Fernando Marinho
Department of Agricultural Sciences, School of Veterinary Medicine, University of Amazonia, Santarém 68040-255, Pará, Brazil
Vanessa dos Santos Morais
Laboratory of Virology (LIM 52), Instituto de Medicina Tropical, Universidade de São Paulo, São Paulo 05403-000, São Paulo, Brazil
Fabiola Villanova
Laboratório de Diversidade Viral, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belem 66075-000, Pará, Brazil
Ramendra Pati Pandey
Centre for Drug Design Discovery and Development (C4D), SRM University, Delhi-NCR, Rajiv Gandhi Education City, Sonepat 131029, Haryana, India
Emerson Luiz Lima Araújo
General Coordination of Public Health, Laboratories of the Strategic Articulation, Department of the Health, Surveillance Secretariat, Ministry of Health (CGLAB/DAEVS/SVS-MS), Brasília 70719-040, Distrito Federal, Brazil
Xutao Deng
General Coordination of Public Health, Laboratories of the Strategic Articulation, Department of the Health, Surveillance Secretariat, Ministry of Health (CGLAB/DAEVS/SVS-MS), Brasília 70719-040, Distrito Federal, Brazil
Eric Delwart
Department Laboratory Medicine, University of California San Francisco, San Francisco, CA 94143, USA
Antonio Charlys da Costa
Laboratory of Virology (LIM 52), Instituto de Medicina Tropical, Universidade de São Paulo, São Paulo 05403-000, São Paulo, Brazil
Elcio Leal
Laboratório de Diversidade Viral, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belem 66075-000, Pará, Brazil
Chaphamaparvovirus (CHPV) is a recently characterized genus of the Parvoviridae family whose members can infect different hosts, including bats, which constitute the second most diverse order of mammals and are described worldwide as important transmitters of zoonotic diseases. In this study, we identified a new CHPV in bat samples from the municipality of Santarém (Pará state, North Brazil). A total of 18 Molossus molossus bats were analyzed using viral metagenomics. In five animals, we identified CHPVs. These CHPV sequences presented the genome with a size ranging from 3797 to 4284 bp. Phylogenetic analysis-based nucleotide and amino acid sequences of the VP1 and NS1 regions showed that all CHPV sequences are monophyletic. They are also closely related to CHPV sequences previously identified in bats in southern and southeast Brazil. According to the International Committee on Taxonomy of Viruses (ICTV) classification criteria for this species (the CHPV NS1 gene region must have 85% identity to be classified in the same species), our sequences are likely a new specie within the genus Chaphamaparvovirus, since they have less than 80% identity with other CHPV described earlier in bats. We also make some phylogenetic considerations about the interaction between CHPV and their host. We suggest a high level of specificity of CPHV and its hosts. Thus, the findings contribute to improving information about the viral diversity of parvoviruses and show the importance of better investigating bats, considering that they harbor a variety of viruses that may favor zoonotic events.
