OncoTargets and Therapy (Mar 2020)
Promotion of miR-221-5p on the Sensitivity of Gastric Cancer Cells to Cisplatin and Its Effects on Cell Proliferation and Apoptosis by Regulating DDR1
Abstract
Xiaomeng Jiang,1 Menglin Jiang,2 Shuhua Guo,3 Pengpeng Cai,1 Wei Wang,1 Yi Li1 1Department of Digestive, Sir Run Run Hospital, Nanjing Medical University, Nanjing, Jiangsu Province, 211166, People’s Republic of China; 2Biomedical Sciences Department, University of Tennessee Health Sciences Center, Memphis, TN 38105, USA; 3Emergency Department, Affiliated Hospital of Jiangsu University, Zhenjiang, Jiangsu Province 212001, People’s Republic of ChinaCorrespondence: Yi LiDepartment of Digestive, Sir Run Run Hospital, Nanjing Medical University, No. 109, Long Mian Avenue, Jiangning District, Nanjing, Jiangsu Province 211166, People’s Republic of ChinaTel +86 18961322162Email [email protected]: This research aimed to explore the role of miR-221-5p on the sensitivity of gastric cancer cells to cisplatin, and the proliferation and invasion of gastric cancer cells by regulating DDR1.Patients and Methods: Altogether 69 patients who treated with radical gastrectomy from January 2014 to January 2016 were collected. With the agree of the patients, 69 gastric carcinoma and 69 adjacent tissues were taken, respectively, during the operation, and gastric carcinoma and human gastric mucosa cells were purchased. RT-PCR was used for detection of the expression level of miR-221-5p and DDR1. Wound healing assay and CCK-8 assay were used for exploration of the cell migration and viability. Western blot and double luciferase reporter gene were performed to determine the target gene of miR-221-5p.Results: It was showed that miR-221-5p expression was decreased in GC tissues and cell lines. The high expression of miR-221-5p reduced the resistance of GC cells to cisplatin and inhibited the proliferation and migration of gastric cancer cells. The high expression of miR-221-5p promoted the proliferation, invasion and migration of GC cells. In addition, we found that DDR1 was a direct target gene of miR-221-5p in GC cells. We found that DDR1 expression increased in gastric carcinoma. Moreover, there was a negative correlation of DDR1 with the expression level of miR-221-5p. The increase of miR-221-5p increased the chemosensitivity of GC cells to cisplatin, and inhibited the proliferation, invasion, migration and EMT of GC cells by targeting DDR1.Conclusion: The above research indicated that miR-221-5p may be a target for enhancing cisplatin chemotherapy sensitivity in gastric cancer patients.Keywords: miR-221-5p, DDR1, gastric cancer cells, cisplatin, sensitivity, proliferation, apoptosis
