Development of simple and fast UV-method for the quantitative determination of mometasone furoate in a large number of metered doses of an aqueous nasal spray of mometasone furoate
Mykhaylo Levin,
Natalia Ostanina,
Oleksii Gumeniuk,
Ruslan Meleshko,
Oksana Tereshchenko,
Yana Nikolaieva,
Vasyl Brytsun,
Nina Tarasenko,
Natalia Savina,
Olena Kuznetsova,
Natalia Ocheretiana,
Anatolii Cheremenko,
Vadym Briazkalo,
Sergii Bykov
Affiliations
Mykhaylo Levin
State Institution “O.M. Marzieiev Institute for Public Health” NAMSU, 50 Popudrenko Street, Kyiv, 02660, Ukraine; Corresponding author.
Natalia Ostanina
State Institution “O.M. Marzieiev Institute for Public Health” NAMSU, 50 Popudrenko Street, Kyiv, 02660, Ukraine
Oleksii Gumeniuk
State Institution “O.M. Marzieiev Institute for Public Health” NAMSU, 50 Popudrenko Street, Kyiv, 02660, Ukraine
Ruslan Meleshko
State Institution “O.M. Marzieiev Institute for Public Health” NAMSU, 50 Popudrenko Street, Kyiv, 02660, Ukraine
Oksana Tereshchenko
National Technical University of Ukraine, Igor Sikorsky Kyiv Polytechnic Institute, Faculty of Chemical Engineering, Ukraine
Yana Nikolaieva
State Institution “O.M. Marzieiev Institute for Public Health” NAMSU, 50 Popudrenko Street, Kyiv, 02660, Ukraine
Vasyl Brytsun
State Institution “O.M. Marzieiev Institute for Public Health” NAMSU, 50 Popudrenko Street, Kyiv, 02660, Ukraine
Nina Tarasenko
State Institution “O.M. Marzieiev Institute for Public Health” NAMSU, 50 Popudrenko Street, Kyiv, 02660, Ukraine
Natalia Savina
State Institution “O.M. Marzieiev Institute for Public Health” NAMSU, 50 Popudrenko Street, Kyiv, 02660, Ukraine
Olena Kuznetsova
State Institution “O.M. Marzieiev Institute for Public Health” NAMSU, 50 Popudrenko Street, Kyiv, 02660, Ukraine
Natalia Ocheretiana
State Institution “O.M. Marzieiev Institute for Public Health” NAMSU, 50 Popudrenko Street, Kyiv, 02660, Ukraine
Anatolii Cheremenko
State Institution “O.M. Marzieiev Institute for Public Health” NAMSU, 50 Popudrenko Street, Kyiv, 02660, Ukraine
Vadym Briazkalo
State Institution “O.M. Marzieiev Institute for Public Health” NAMSU, 50 Popudrenko Street, Kyiv, 02660, Ukraine
Sergii Bykov
State Institution “O.M. Marzieiev Institute for Public Health” NAMSU, 50 Popudrenko Street, Kyiv, 02660, Ukraine
Fast and simple spectrophotometric method for quantitative determination of mometasone furoate in a single dose (single actuation) of its nasal spray was developed and validated. This method is based on the spectrophotometric analysis of turbid solution of a single spray in 20 mL isopropanol at 220–310 nm. We further show applicability of this method for analysis of large number of single sprays for the dosing homogeneity. It is based on a new approach to the development of spectrophotometric quantitative determination methods for drug products and possibly other objects, allowing measurements to be carried out on sufficiently turbid test solutions. The proposed approach is not a variant of the derivative spectrophotometry and can replace the methods of derivative spectrophotometry in cases where: derivative of analyte spectrum is not intense enough; derivative of the spectrum of the matrix (turbidity and remaining components of the product) is significant when compared with the derivative of the analyte.
