Antioxidant and Anticholinesterase Properties of Sideritis perfoliata subsp. athoa (Papan. &Kokkini) Baden and Sideritis trojana Bornm. Teas from Mount Ida-Turkey and Their Phenolic Characterization by LC-MS/MS

Abstract

The phenolic profile, antioxidant and anticholinesterase activities of teas prepared from two Sideritis(L.) species: Sideritis perfoliata subsp. athoa (Papan. &Kokkini) Baden. and Sideritis trojana Bornm, collected from Mount Ida (Kaz Dağı) Turkey, were determined. The teas were prepared by infusion and decoction methods. The quantitative amounts of the phenolic contents were determined by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The major phytochemicals were found to be fumaric acid for infusion and decoction samples of S. trojana and infusion of S. perfoliata subsp. athoa, chlorogenic acid for decoction of S. perfoliata subsp. athoa. The tea samples prepared by decoction method were found to be rich in phenolics. Apart from the fact that the decoction sample of S. trojana exhibited the best antioxidant effect in 2, 2-diphenyl-1-picrylhydrazyl (DPPH), β -carotene bleaching and cupric (Cu2+) ion reducing power assay (CUPRAC) methods among the tested samples at all concentrations, and showed significant inhibition effect at 200 μg/mL against acetylcholinesterase and butyrylcholinesterase enzymes (59.74%, 64.99%, respectively).

Keywords

• sideritis
• sideritis perfoliata subsp. athoa (papan. &kokkini) baden.
• sideritis trojana bornm
• antioxidant activity
• anticholinesterase activity
• phenolics