INFLUENCE OF OXYGENATOR EXTRACORPORAL CIRCUIT TREATMENT WITH ADAPTATION COMPOSITION (AdC) ON MORPHOLOGICAL CHANGES OF ERYTHROCYTES.

Abstract

Summary. The study highlights a method of treatment extracorporeal circuit with «adaptation composition» (AdC) for the reduction of negative impact on state of erythrocytes. Materials and methods. A total of 90 patients were enrolled, they were divided into two groups. The group 1 (45 patients, 39/6 male/female) included patients who underwent surgical procedures without treatment of an extracorporeal circuit with AdC. The group 2 (45 patients, 39/6 male/female) included patients who underwent surgery with the treatment of an extracorporeal circuit with AdC. According to the study protocol, patient blood was sampling for complete blood cell count (CBC) and erythrocyte morphology at 4 stages of surgery: before surgery, at 10 min. CPB-time, at 60 min. CPB-time and after separation from CPB. Results. The albumin of AdC creates a protective nanolayer on the surface of the oxygenator membrane and tubes. There were no statistically significant differences of parameters in the groups before CPB. Level of Ht 2 (group 2) at 60 min CPB-time and after CPB, were lower than Ht 1 (group 1) (p=0.021 and p=0.035 correspondingly) because MCV1 was higher (р=0.025 and p<0.0001 correspondingly). The increase MCHC in groups at 10 min. CPB-time relatives with the decrease in MCV at 10 min CPB-time. At 60 min, there are changes of RDWa2 76.05 ± 5.46 and RDWa1 72.35 ± 7.26, p<0.000. After CPB higher content of reticulocytes (р <0.0001), echinocytes (р <0.0001) and spherocytes (р <0.0001) is observed in group 1. The lowering of mechanical resistance (р = 0.04) and increasing membrane permeability for urea were in group 1. After CPB the best aсid hemolysis resistance was in group 2 (р = 0.05), erythrocytes were more resistant to hypoosmotic factor (р = 0.01) in group 2. Conclusion. The treatment of oxygenator with AdC reduces the negative influence СРВ on state of RBC. Membranes of erythrocytes were more resistant to traumatic factors in the group with AdC.

Keywords

• cardiopulmonary bypass (cpb), oxygenator, erythrocyte morphology, adaptation composition.