Non-flipping DNA glycosylase AlkD scans DNA without formation of a stable interrogation complex

Arash Ahmadi; Katharina Till; Paul Hoff Backe; Pernille Blicher; Robin Diekmann; Mark Schüttpelz; Kyrre Glette; Jim Tørresen; Magnar Bjørås; Alexander D. Rowe; Bjørn Dalhus

doi:10.1038/s42003-021-02400-x

Communications Biology (Jul 2021)

Non-flipping DNA glycosylase AlkD scans DNA without formation of a stable interrogation complex

Arash Ahmadi,
Katharina Till,
Paul Hoff Backe,
Pernille Blicher,
Robin Diekmann,
Mark Schüttpelz,
Kyrre Glette,
Jim Tørresen,
Magnar Bjørås,
Alexander D. Rowe,
Bjørn Dalhus

Affiliations

Arash Ahmadi: Department of Medical Biochemistry, Institute for Clinical Medicine, University of Oslo
Katharina Till: FOM Institute AMOLF, Science Park 104
Paul Hoff Backe: Department of Medical Biochemistry, Institute for Clinical Medicine, University of Oslo
Pernille Blicher: Department of Medical Biochemistry, Institute for Clinical Medicine, University of Oslo
Robin Diekmann: Biomolecular Photonics, Department of Physics, University of Bielefeld
Mark Schüttpelz: Biomolecular Photonics, Department of Physics, University of Bielefeld
Kyrre Glette: Department of Informatics, University of Oslo
Jim Tørresen: Department of Informatics, University of Oslo
Magnar Bjørås: Department of Microbiology, Oslo University Hospital HF, Rikshospitalet and University of Oslo
Alexander D. Rowe: Department of Medical Biochemistry, Institute for Clinical Medicine, University of Oslo
Bjørn Dalhus: Department of Medical Biochemistry, Institute for Clinical Medicine, University of Oslo

DOI: https://doi.org/10.1038/s42003-021-02400-x
Journal volume & issue: Vol. 4, no. 1
pp. 1 – 8

Abstract

Read online

Ahmadi et al. use a single-molecule tracking method to describe the DNA scanning mode of AlkD, a HEAT-like repeat DNA glycosylase. They show that, contrary to other glycosylases that use a base-flipping mechanism, AlkD scans the DNA without forming a stable interrogation complex.

Published in Communications Biology

ISSN: 2399-3642 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science: Biology (General)
Website: https://www.nature.com/commsbio/

About the journal