Pooled CRISPR-activation screening coupled with single-cell RNA-seq in mouse embryonic stem cells

Abstract

Summary: CRISPR/Cas9 screens are a powerful approach to identify key regulators of biological processes. By combining pooled CRISPR/Cas9 screening with single-cell RNA-sequencing readout, individual perturbations can be assessed in parallel both comprehensively and at scale. Importantly, this allows gene function and regulation to be interrogated at a cellular level in an unbiased manner. Here, we present a protocol to perform pooled CRISPR-activation screens in mouse embryonic stem cells using 10× Genomics scRNA-seq as a readout.For complete information on the generation and use of this protocol, please refer to Alda-Catalinas et al. (2020).

Keywords

• Cell culture
• Single Cell
• Sequencing
• RNAseq
• High Throughput Screening
• Gene Expression