Communications Biology (Jan 2022)

Improved clearing method contributes to deep imaging of plant organs

  • Yuki Sakamoto,
  • Anna Ishimoto,
  • Yuuki Sakai,
  • Moeko Sato,
  • Ryuichi Nishihama,
  • Konami Abe,
  • Yoshitake Sano,
  • Teiichi Furuichi,
  • Hiroyuki Tsuji,
  • Takayuki Kohchi,
  • Sachihiro Matsunaga

DOI
https://doi.org/10.1038/s42003-021-02955-9
Journal volume & issue
Vol. 5, no. 1
pp. 1 – 12

Abstract

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Sakamoto et al. demonstrate an improved optical clearing method, iTOMEI, for plant imaging. The new method can achieve fast clearing and effective removal of autofluorescence signals, and at the same time preserve signals from desired fluorescence proteins.