Encapsulated Brucella ovis Lacking a Putative ATP-Binding Cassette Transporter (ΔabcBA) Protects against Wild Type Brucella ovis in Rams.

PLoS ONE. 2015;10(8):e0136865 DOI 10.1371/journal.pone.0136865


Journal Homepage

Journal Title: PLoS ONE

ISSN: 1932-6203 (Online)

Publisher: Public Library of Science (PLoS)

LCC Subject Category: Medicine | Science

Country of publisher: United States

Language of fulltext: English

Full-text formats available: PDF, HTML, XML



Ana Patrícia C Silva
Auricélio A Macêdo
Luciana F Costa
Cláudia E Rocha
Luize N N Garcia
Jade R D Farias
Priscilla P R Gomes
Gustavo C Teixeira
Kessler W J Fonseca
Andréa R F Maia
Gabriela G Neves
Everton L Romão
Teane M A Silva
Juliana P S Mol
Renata M Oliveira
Márcio S S Araújo
Ernane F Nascimento
Olindo A Martins-Filho
Humberto M Brandão
Tatiane A Paixão
Renato L Santos


Peer review

Editorial Board

Instructions for authors

Time From Submission to Publication: 24 weeks


Abstract | Full Text

This study aimed to evaluate protection induced by the vaccine candidate B. ovis ΔabcBA against experimental challenge with wild type B. ovis in rams. Rams were subcutaneously immunized with B. ovis ΔabcBA encapsulated with sterile alginate or with the non encapsulated vaccine strain. Serum, urine, and semen samples were collected during two months after immunization. The rams were then challenged with wild type B. ovis (ATCC25840), and the results were compared to non immunized and experimentally challenged rams. Immunization, particularly with encapsulated B. ovis ΔabcBA, prevented infection, secretion of wild type B. ovis in the semen and urine, shedding of neutrophils in the semen, and the development of clinical changes, gross and microscopic lesions induced by the wild type B. ovis reference strain. Collectively, our data indicates that the B. ovis ΔabcBA strain is an exceptionally good vaccine strain for preventing brucellosis caused by B. ovis infection in rams.