Synthesis of Cerium Oxide Nanoparticles in a Bacterial Nanocellulose Matrix and the Study of Their Oxidizing and Reducing Properties
Nina Melnikova,
Darina Malygina,
Vitaly Korokin,
Hayder Al-Azzawi,
Daria Zdorova,
Evgeniy Mokshin,
Elena Liyaskina,
Irina Kurgaeva,
Victor Revin
Affiliations
Nina Melnikova
Faculty of Chemistry, Lobachevsky University, 23/5 Gagarin Av., Nizhny Novgorod 603950, Russia
Darina Malygina
Department of Pharmaceutical Chemistry, Privolzhsky Research Medical University, 10/1 Minin Sq., Nizhny Novgorod 603950, Russia
Vitaly Korokin
Faculty of Chemistry, Lobachevsky University, 23/5 Gagarin Av., Nizhny Novgorod 603950, Russia
Hayder Al-Azzawi
Faculty of Chemistry, Lobachevsky University, 23/5 Gagarin Av., Nizhny Novgorod 603950, Russia
Daria Zdorova
Faculty of Chemistry, Lobachevsky University, 23/5 Gagarin Av., Nizhny Novgorod 603950, Russia
Evgeniy Mokshin
Department of Biotechnology, Bioengineering and Biochemistry, National Research Ogarev Mordovia State University, 68 Bolshevistskaya str., Saransk 430005, Russia
Elena Liyaskina
Department of Biotechnology, Bioengineering and Biochemistry, National Research Ogarev Mordovia State University, 68 Bolshevistskaya str., Saransk 430005, Russia
Irina Kurgaeva
Department of Biotechnology, Bioengineering and Biochemistry, National Research Ogarev Mordovia State University, 68 Bolshevistskaya str., Saransk 430005, Russia
Victor Revin
Department of Biotechnology, Bioengineering and Biochemistry, National Research Ogarev Mordovia State University, 68 Bolshevistskaya str., Saransk 430005, Russia
A soft synthesis of nanoceria with non-stoichiometric composition (33% Ce3+/67% Ce4+) named CeO2 NPs in bacterial cellulose (BC) matrix in the form of aerogel and hydrogel with controlled CeO2 NPs content was proposed. The advantage of CeO2 NPs synthesis in BC is the use of systemic antacid API–trisamine as a precursor, which did not destruct cellulose at room temperature and enabled a reduction in the duration of synthesis and the number of washes. Moreover, this method resulted in the subsequent uniform distribution of CeO2 NPs in the BC matrix due to cerium (III) nitrate sorption in the BC matrix. CeO2 NPs (0.1–50.0%) in the BC matrix had a fluorite structure with a size of 3–5 nm; the specific surface area of the composites was 233.728 m2/g. CeO2 NPs in the BC-CeO2 NPs composite demonstrated SOD-like activity in the processes of oxidation and reduction of cytochrome c (cyt c3+/cyt c2+), as well as epinephrine to inhibit its auto-oxidation in aqueous solutions by 33–63% relative to the control. In vitro experiments on rat blood showed a decrease in the MDA level and an increase in the activity of antioxidant defense enzymes–SOD by 24% and G6PDH by 2.0–2.5 times. Therefore, BC-CeO2 NPs can be proposed for wound healing as antioxidant material.
