Bioingeniøren (Aug 2015)
p16INK4a and Ki-67 immunostaining - an alternative to rescreening of cervical samples with normal cytology and positive HPV test
Abstract
Background: Double immunostaining with CINtec® PLUS can be used to detect the proteins p16INK4a and Ki-67 in cytological cervix preparations. Detection of both proteins in the same cell is an indication of deregulated cell cycle and possible precancerous lesions. The purpose of the study was to determine if double immunostaining of p16INK4a and Ki-67 could be used for localization of cells that could represent precancerous lesions of the cervix in women with normal Pap smear and positive HPV DNA test. Material and methods: 25 cervical specimens, assessed as cytological normal and tested HPV DNA positive, were used. The method was double immunostaining of p16INK4a and Ki-67 with use of CINtec® PLUS. Results: 10 of 25 samples were positive by the double immunostaining, 14 were negative and one was considered uncertain. 4 of the 10 positive samples contained abnormal cells by cytological evaluation. These were diagnosed ASC-US and LSIL. Conclusion: In 4 of 25 samples there were found low-grade cervical dysplasia. Double immunostaining of p16INK4a and Ki-67 can be used to find cells with abnormalities in samples assessed as cytological normal and tested HPV DNA positive.
