International Journal of Nanomedicine (Mar 2023)

Diagnosis of Toxoplasmosis Using Surface Antigen Grade 1 Detection by ELISA, Nano-Gold ELISA, and PCR in Pregnant Women

  • Aly NS,
  • Kim HS,
  • Marei YM,
  • Elhamshary AS,
  • Bayoumi IR,
  • Omar RE,
  • Mohammed DA,
  • Miyoshi SI,
  • Rashed GA

Journal volume & issue
Vol. Volume 18
pp. 1335 – 1345

Abstract

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Nagwa SM Aly,1,2 Hye-Sook Kim,2 Yasmin M Marei,3 Azza S Elhamshary,1 Ibrahim R Bayoumi,4 Rabab E Omar,1 Dina A Mohammed,1 Shin-Ichi Miyoshi,5 Gehan A Rashed1 1Department of Parasitology, Benha Faculty of Medicine, Benha University, Benha, 13511, Egypt; 2Division of International Infectious Diseases Control, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, 700-8530, Japan; 3Medical Biochemistry Department, Faculty of Medicine, Benha University, Benha, 13511, Egypt; 4Parasitology Department, Theodor Bilharz Research Institute, Giza, Egypt; 5Department of Sanitary Microbiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, 700-8530, JapanCorrespondence: Nagwa SM Aly, Department of Parasitology, Benha Faculty of Medicine, Benha University, Fareed Nada Street, Benha, 13511, Egypt, Tel +2-13-9101186, Email [email protected]: The accurate diagnosis of toxoplasmosis has critical importance in pregnant women. Nanotechnology and molecular biology are making possible opportunities for accurate and rapid diagnosis of many infectious diseases.Aim and Methods: The aim of our study was to compare nano-gold ELISA with ELISA and PCR for diagnosis of toxoplasmosis using Toxoplasma surface antigen grade 1 (SAG1) in pregnant women seeking antenatal care in outpatient clinics.Results: PCR showed the highest diagnostic values than nano-gold ELISA and ELISA regarding sensitivity (97.3% versus 89.2% and 83.8%); specificity (100% versus 94% and 88%); and diagnostic accuracy (98.9% versus 91.95% and 86.2%), respectively. There is no statistical difference between PCR and nanogold ELISA results.Discussion: Nano-gold ELISA had a significant improvement in diagnosis than the traditional ELISA method. Most likely with the assistance of nanoparticles, more antibodies enter the antigen–antibody complex because of the considerable improvement in the surface area of nano-gold particles.Conclusion: Although PCR had higher diagnostic values than nano ELISA, nano ELISA is cheaper and easier than PCR. We recommend nano-gold ELISA with SAG1 as a promising technique in the diagnosis of toxoplasmosis and survey studies.Keywords: toxoplasmosis, SAG 1, immunodiagnosis, nanogold, ELISA, PCR

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