Triphasic developmentally guided protocol to generate retinal pigment epithelium from induced pluripotent stem cells

Abstract

Summary: RPE tissues are derived from induced pluripotent stem cells (iPSCs) to model retinal diseases and as a replacement therapy for macular degeneration. Here, we developed a robust and efficient directed differentiation protocol to generate pure RPE cells that form a polarized monolayer. This protocol describes how to set up RPE differentiation and to obtain a pure population that expresses mature RPE markers and forms strong tight junctions.For complete details on the use and execution of this protocol, please refer to Sharma et al. (2019, 2021) and Miyagishima et al. (2021). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Keywords

• Cell Biology
• Cell culture
• Cell isolation
• Flow Cytometry/Mass Cytometry
• Health Sciences
• Stem Cells