Ciência Rural ()

Osteoprogenitor cells can enhance early bone formation in critical bone defects in dogs

  • Endrigo Gabellini Leonel Alves,
  • Rogéria Serakides,
  • Isabel Rodrigues Rosado,
  • Omar Leonardo Aristizabal Paez,
  • Jéssica Alejandra Castro Varon,
  • Felipe Nemer Machado,
  • Fabíola Bono Fukushima,
  • Alfredo Miranda Góes,
  • Cleuza Maria de Faria Rezende

DOI
https://doi.org/10.1590/0103-8478cr20151109
Journal volume & issue
Vol. 47, no. 7

Abstract

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ABSTRACT: The aim of this study was to evaluate the effect of osteoprogenitor cells derived from mesenchymal stem cells from adipose tissue (OC-AD-MSCs), and differentiated into osteoblasts, in the treatment of critical bone defects in dogs. Adipose tissue derived mesenchymal stem cells (AD-MSCs) were subjected to osteogenic differentiation for 21 days and used in the treatment of bone defects in dogs radius. Either three experimental groups were bone defects treated with OC-AD-MSCs (OC), defects filled with autogenous bone (Control- C +), or empty defects (Control- C -). Bone regeneration was assessed by radiology, densitometry, and histomorphometry. The area of new bone formation was higher in the OC group compared to the control group (C-) on postoperative day 15. Defects treated with OC-AD-MSCs showed greater neovascularization than the other two groups at 90 days. We concluded that treatment with OC-AD-MSCs increased the area of new bone formation 15 days after surgery; however, it didn’t complete the bone union in critical bone defects in the radius of dogs at 90 days.

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