Effects of the Fhb1 gene on Fusarium head blight resistance and agronomic traits of winter wheat
Teng Li,
Hongjun Zhang,
Yiwen Huang,
Zhenqi Su,
Yun Deng,
Hongwei Liu,
Chunyan Mai,
Guangjun Yu,
Huili Li,
Liqiang Yu,
Tongquan Zhu,
Li Yang,
Hongjie Li,
Yang Zhou
Affiliations
Teng Li
National Engineering Laboratory for Crop Molecular Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Hongjun Zhang
National Engineering Laboratory for Crop Molecular Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Yiwen Huang
National Engineering Laboratory for Crop Molecular Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Zhenqi Su
Institute of Cereal and Oil Crops, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050035, Hebei, China
Yun Deng
Nanping Institute of Agricultural Sciences of Fujian Province, Jianyang 354200, Fujian, China
Hongwei Liu
National Engineering Laboratory for Crop Molecular Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Chunyan Mai
Xinxiang Innovation Center for Breeding Technology of Dwarf-Male-Sterile Wheat, Xinxiang 453731, Henan, China
Guangjun Yu
Zhaoxian Experiment Station, Shijiazhuang Academy of Agricultural and Forestry Sciences, Zhaoxian 051530, Hebei, China
Huili Li
Zhaoxian Experiment Station, Shijiazhuang Academy of Agricultural and Forestry Sciences, Zhaoxian 051530, Hebei, China
Liqiang Yu
Zhaoxian Experiment Station, Shijiazhuang Academy of Agricultural and Forestry Sciences, Zhaoxian 051530, Hebei, China
Tongquan Zhu
Wheat Research Institute, Zhumadian Academy of Agricultural Sciences, Zhumadian 463000, Henan, China
Li Yang
National Engineering Laboratory for Crop Molecular Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Hongjie Li
National Engineering Laboratory for Crop Molecular Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China; Corresponding authors.
Yang Zhou
National Engineering Laboratory for Crop Molecular Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China; Corresponding authors.
The gene Fhb1 has been used in many countries to improve wheat Fusarium head blight (FHB) resistance. To make better use of this gene in the Yellow-Huai River Valleys Winter Wheat Zone (YHWZ), the most important wheat-producing region of China, it is desirable to elucidate its effects on FHB resistance and agronomic traits in different genetic backgrounds. Based on a diagnostic marker for Fhb1, six BC2 populations were developed by crossing dwarf-male-sterile (DMS)-Zhoumai 16 to three Fhb1 donors (Ningmai 9, Ningmai 13, and Jianyang 84) and backcrossing to Zhoumai 16 and Zhoumai 16's derivative cultivars (Lunxuan 136 and Lunxuan 13) using marker-assisted backcross breeding. The progenies were assessed for FHB resistance and major agronomic traits. The Fhb1 alleles were identified using the gene-specific molecular marker. The plants with the Fhb1-resistant genotype (Fhb1-R) in these populations showed significantly fewer infected spikelets than those with the Fhb1-susceptible genotype (Fhb1-S). When Lunxuan 136 was used as the recurrent parent, Fhb1-R plants showed significantly fewer infected spikelets per spike than Fhb1-R plants produced using Lunxuan 13 as the recurrent parent, indicating that the genetic backgrounds of Fhb1 influence the expression of FHB resistance. Fhb1-R plants from the DMS-Zhoumai 16/Ningmai 9//Zhoumai 16/3/Lunxuan 136 population showed the highest FHB resistance among the six populations and a significantly higher level of FHB resistance than the moderately susceptible control Huaimai 20. No significant phenotypic differences between Fhb1-R and Fhb1-S plants were observed for the eight agronomic traits investigated. These results suggest that it is feasible to improve FHB resistance of winter wheat without reducing yield potential by introgressing Fhb1 resistance allele into FHB-susceptible cultivars in the YHWZ. Keywords: Triticum aestivum L., Fhb1 gene, Marker-assisted selection, Fusarium head blight resistance, Agronomic traits
