Peculiairities of mRNA TLR-2, TLR-4 expression of the oral cavity epithelium in children under conditions of chronic catarrhal gingivitis and diabetes mellitus

Abstract

TLRs in Epithelium of the oral cavity can be effected as due to the dental as general somatic pathology. TLR availability in the external membrane of neutrophils, macrophages, keratocytes was found to be a starting point in triggering inflammation in the periodontal tissues ensuring molecular reception of a pathogen with further involvement of important components in the inherited immunity. These effectors possess phagocytic and killer activities, ensure a number of signals activating and directing antigen-specific response by the cells of the adaptive immune system. The aim of the study was to study mRNA TLR-2, TLR-4 in the epithelium of the oral cavity in children with chronic catarrhal gingivitis and diabetes mellitus taking to account revealed metabolic disorders. Materials and methods. 30 children with chronic catarrhal gingivitis and diabetes mellitus type I were included into Іst group, 30 children with chronic catarrhal gingivitis were included into the ІInd group and 30 absolutely healthy children -ІII group. Polymerase chain reaction with reverse transcription in the regime in real time (RT-RRT) was used to analyze gene expression. Buccal epithelium was used as an object for molecular-genetic study. Results. Content of mRNA TLR-2 (90.0755) in diabetes mellitus patients was in 5.5 times higher as compared to children with CCG – 15.1505. Expression of mRNA TLR-4 in children of I group increased in 6 times as compared to the children from II group. Conclusion. Relative quantity of mRNA TLR-2 and TLR-4 in the epithelium of the oral cavity in children with diabetes mellitus was considerably higher than in patients without chronic inflammatory disease.

Keywords

• type I diabetes mellitus
• gingivitis
• TLR