Altered B cell immunoglobulin signature exhibits potential diagnostic values in human colorectal cancer
Rui-Xian Liu,
Chuangyu Wen,
Weibiao Ye,
Yewei Li,
Junxiong Chen,
Qian Zhang,
Weiqian Li,
Wanfei Liang,
Lili Wei,
Jingdan Zhang,
Ka-Wo Chan,
Xueqin Wang,
Xiangling Yang,
Huanliang Liu
Affiliations
Rui-Xian Liu
Department of Clinical Laboratory, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong 510655, China; Guangdong Institute of Gastroenterology, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong 510655, China; Guangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Diseases, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong 510655, China
Chuangyu Wen
Affiliated Dongguan Hospital, Southern Medical University, Dongguan, Guangdong 523059, China
Weibiao Ye
Affiliated Dongguan Hospital, Southern Medical University, Dongguan, Guangdong 523059, China
Yewei Li
Department of Statistical Science, School of Mathematics, Sun Yat-sen University, Guangzhou, Guangdong 510275, China
Junxiong Chen
Guangdong Institute of Gastroenterology, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong 510655, China
Qian Zhang
Guangdong Institute of Gastroenterology, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong 510655, China
Weiqian Li
Guangdong Institute of Gastroenterology, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong 510655, China
Wanfei Liang
Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, Guangdong 510080, China
Lili Wei
Department of Clinical Laboratory, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong 510655, China
Jingdan Zhang
Department of Clinical Laboratory, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong 510655, China
Ka-Wo Chan
Guangdong Institute of Gastroenterology, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong 510655, China
Xueqin Wang
International Institute of Finance, School of Management, University of Science and Technology of China, Hefei, Anhui 230026, China
Xiangling Yang
Guangdong Institute of Gastroenterology, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong 510655, China; Guangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Diseases, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong 510655, China; Corresponding author
Huanliang Liu
Department of Clinical Laboratory, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong 510655, China; Guangdong Institute of Gastroenterology, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong 510655, China; Guangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Diseases, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong 510655, China; Corresponding author
Summary: Antibody-secreting B cells have long been considered the central element of gut homeostasis; however, tumor-associated B cells in human colorectal cancer (CRC) have not been well characterized. Here, we show that the clonotype, phenotype, and immunoglobulin subclasses of tumor-infiltrating B cells have changed compared to adjacent normal tissue B cells. Remarkably, the tumor-associated B cell immunoglobulin signature alteration can also be detected in the plasma of patients with CRC, suggesting that a distinct B cell response was also evoked in CRC. We compared the altered plasma immunoglobulin signature with the existing method of CRC diagnosis. Our diagnostic model exhibits improved sensitivity compared to the traditional biomarkers, CEA and CA19-9. These findings disclose the altered B cell immunoglobulin signature in human CRC and highlight the potential of using the plasma immunoglobulin signature as a non-invasive method for the assessment of CRC.
