Wnt and Neuregulin1/ErbB signalling extends 3D culture of hormone responsive mammary organoids

Thierry Jardé; Bethan Lloyd-Lewis; Mairian Thomas; Howard Kendrick; Lorenzo Melchor; Lauriane Bougaret; Peter D. Watson; Kenneth Ewan; Matthew J. Smalley; Trevor C. Dale

doi:10.1038/ncomms13207

Nature Communications (Oct 2016)

Wnt and Neuregulin1/ErbB signalling extends 3D culture of hormone responsive mammary organoids

Thierry Jardé,
Bethan Lloyd-Lewis,
Mairian Thomas,
Howard Kendrick,
Lorenzo Melchor,
Lauriane Bougaret,
Peter D. Watson,
Kenneth Ewan,
Matthew J. Smalley,
Trevor C. Dale

Affiliations

Thierry Jardé: Cardiff School of Biosciences, Cardiff University
Bethan Lloyd-Lewis: Cardiff School of Biosciences, Cardiff University
Mairian Thomas: Cardiff School of Biosciences, Cardiff University
Howard Kendrick: European Cancer Stem Cell Research Institute, Cardiff School of Biosciences, Cardiff University
Lorenzo Melchor: Division of Breast Cancer Research, Breast Cancer Now, Institute of Cancer Research
Lauriane Bougaret: Cardiff School of Biosciences, Cardiff University
Peter D. Watson: Cardiff School of Biosciences, Cardiff University
Kenneth Ewan: Cardiff School of Biosciences, Cardiff University
Matthew J. Smalley: European Cancer Stem Cell Research Institute, Cardiff School of Biosciences, Cardiff University
Trevor C. Dale: Cardiff School of Biosciences, Cardiff University

DOI: https://doi.org/10.1038/ncomms13207
Journal volume & issue: Vol. 7, no. 1
pp. 1 – 14

Abstract

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Three-dimensional culture systems and organoids for mammary glands are important to understand mammary gland development. Here, the authors identify conditions (including Neuregulin 1 and R-spondin 1) that allow the culture of organoids that are responsive to hormonal stimulation for up to 2.5 months.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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