International Journal of Nanomedicine (Oct 2019)
Enhanced anti-tumor immunotherapy by silica-coated magnetic nanoparticles conjugated with ovalbumin
Abstract
Sung-Ju Lee,1 Jong-Jin Kim,2 Kyung-Yun Kang,3 Man-Jeong Paik,1 Gwang Lee,4 Sung-Tae Yee1 1Department of Pharmacy, Sunchon National University, Suncheon 540-742, Republic of Korea; 2Center for Self-assembly and Complexity, Institute for Basic Science (IBS), Pohang 37673, Republic of Korea; 3Suncheon Research Center for Natural Medicines, Suncheon, Republic of Korea; 4Department of Physiology and Department of Biomedical Science, Ajou University School of Medicine, Suwon, Republic of KoreaCorrespondence: Sung-Tae YeeDepartment of Pharmacy, Sunchon National University, 255 Joongang-Ro, Seokhyeon-Dong, Suncheon 549-742, Republic of KoreaTel +82 61 750 3752Fax +82 61 750 3708Email [email protected]: The effective induction of an antigen-specific T cell immune response through dendritic cell activation is one of the key goals of tumor immunotherapy.Methods: In this study, efficient antigen-delivery carriers using silica-coated magnetic nanoparticles were designed and, their antigen-specific T cell immune response through dendritic cell activation investigated.Results: The results showed that the silica-coated magnetic nanoparticles with conjugated ovalbumin enhanced the production of cytokines and antigen uptake in bone marrow-derived dendritic cells. Also, this induced an antigen-specific cytotoxic T lymphocyte (CTL) immune response and activated antigen-specific Th1 cell responses, including IL-2 and IFN-γ production and proliferation. We proved that the immune-stimulatory effects of silica-coated magnetic nanoparticles with conjugated ovalbumin were efficient in inhibiting of tumor growth in EG7-OVA (mouse lymphoma-expressing ovalbumin tumor-bearing mice model).Conclusion: Therefore, the silica-coated magnetic nanoparticles with conjugated ovalbumin are expected to be useful as efficient anti-cancer immunotherapy agents.Keywords: antigen-delivery systems, silica-coated magnetic nanoparticles, ovalbumin, EG-7, dendritic cell, CD4+ T cell, CD8+ T cell
