Improved Productivity of Streptomyces mobaraensis Transglutaminase by Regulating Zymogen Activation

Xiaoqiang Yin; Xiaoqiang Yin; Xiaoqiang Yin; Shengqi Rao; Jingwen Zhou; Jingwen Zhou; Jingwen Zhou; Jingwen Zhou; Guocheng Du; Guocheng Du; Guocheng Du; Jian Chen; Jian Chen; Jian Chen; Song Liu; Song Liu; Song Liu

doi:10.3389/fbioe.2022.878795

Frontiers in Bioengineering and Biotechnology (Apr 2022)

Improved Productivity of Streptomyces mobaraensis Transglutaminase by Regulating Zymogen Activation

Xiaoqiang Yin,
Xiaoqiang Yin,
Xiaoqiang Yin,
Shengqi Rao,
Jingwen Zhou,
Jingwen Zhou,
Jingwen Zhou,
Jingwen Zhou,
Guocheng Du,
Guocheng Du,
Guocheng Du,
Jian Chen,
Jian Chen,
Jian Chen,
Song Liu,
Song Liu,
Song Liu

Affiliations

Xiaoqiang Yin: National Engineering Research Center for Cereal Fermentation and Food Biomanufacturing, Jiangnan University, Wuxi, China
Xiaoqiang Yin: Science Center for Future Foods, Jiangnan University, Wuxi, China
Xiaoqiang Yin: School of Biotechnology, Jiangnan University, Wuxi, China
Shengqi Rao: College of Food Science and Engineering, Yangzhou University, Yangzhou, China
Jingwen Zhou: National Engineering Research Center for Cereal Fermentation and Food Biomanufacturing, Jiangnan University, Wuxi, China
Jingwen Zhou: Science Center for Future Foods, Jiangnan University, Wuxi, China
Jingwen Zhou: School of Biotechnology, Jiangnan University, Wuxi, China
Jingwen Zhou: Jiangsu Provincial Engineering Research Center for Bioactive Product Processing, Jiangnan University, Wuxi, China
Guocheng Du: National Engineering Research Center for Cereal Fermentation and Food Biomanufacturing, Jiangnan University, Wuxi, China
Guocheng Du: Science Center for Future Foods, Jiangnan University, Wuxi, China
Guocheng Du: School of Biotechnology, Jiangnan University, Wuxi, China
Jian Chen: National Engineering Research Center for Cereal Fermentation and Food Biomanufacturing, Jiangnan University, Wuxi, China
Jian Chen: Science Center for Future Foods, Jiangnan University, Wuxi, China
Jian Chen: School of Biotechnology, Jiangnan University, Wuxi, China
Song Liu: National Engineering Research Center for Cereal Fermentation and Food Biomanufacturing, Jiangnan University, Wuxi, China
Song Liu: Science Center for Future Foods, Jiangnan University, Wuxi, China
Song Liu: School of Biotechnology, Jiangnan University, Wuxi, China

DOI: https://doi.org/10.3389/fbioe.2022.878795
Journal volume & issue: Vol. 10

Abstract

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Streptomyces mobaraensis transglutaminase (TGase) is extracellularly expressed as a zymogen and then activated by TGase-activating protease (TAP). In this study, we reported the strategy for improving TGase production via the regulation of TAP activity in S. mobaraensis. First, we analyzed the effects of three inorganic nitrogen sources on TGase production. With 30 mM nitrogen content, the time to the peak of TGase activity induced by (NH4)2SO4 or NH4Cl was 72 h, 12 h earlier than that of the fermentation without adding NH4+. SDS-PAGE analysis indicated that NH4+ accelerated the TGase activation in S. mobaraensis. Then, we examined the effect of NH4+ on TAP biosynthesis using a TGase-deficient S. mobaraensis strain. It showed that NH4+ enhanced the TAP activity at the early stage of the fermentation, which was dependent on the concentration and time of NH4+ addition. Last, the yield and productivity of S. mobaraensis TGase were increased by 1.18-fold and 2.1-fold, respectively, when optimal NH4+ addition (60 mM and 12 h) was used. The fermentation period was shortened from 84 to 48 h. The NH4+ addition also increased the storage stability of crude enzyme at room temperature. These findings will benefit the TGase production and its activation mechanism in S. mobaraensis.

Published in Frontiers in Bioengineering and Biotechnology

ISSN: 2296-4185 (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Technology: Chemical technology: Biotechnology
Website: http://www.frontiersin.org/bioengineering_and_biotechnology

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