Cryo-EM structure of the human MLL1 core complex bound to the nucleosome

Sang Ho Park; Alex Ayoub; Young-Tae Lee; Jing Xu; Hanseong Kim; Wei Zheng; Biao Zhang; Liang Sha; Sojin An; Yang Zhang; Michael A. Cianfrocco; Min Su; Yali Dou; Uhn-Soo Cho

doi:10.1038/s41467-019-13550-2

Nature Communications (Dec 2019)

Cryo-EM structure of the human MLL1 core complex bound to the nucleosome

Sang Ho Park,
Alex Ayoub,
Young-Tae Lee,
Jing Xu,
Hanseong Kim,
Wei Zheng,
Biao Zhang,
Liang Sha,
Sojin An,
Yang Zhang,
Michael A. Cianfrocco,
Min Su,
Yali Dou,
Uhn-Soo Cho

Affiliations

Sang Ho Park: Department of Biological Chemistry, University of Michigan
Alex Ayoub: Department of Pathology, University of Michigan
Young-Tae Lee: Department of Pathology, University of Michigan
Jing Xu: Department of Pathology, University of Michigan
Hanseong Kim: Department of Biological Chemistry, University of Michigan
Wei Zheng: Computational Medicine and Bioinformatics, University of Michigan
Biao Zhang: Computational Medicine and Bioinformatics, University of Michigan
Liang Sha: Department of Pathology, University of Michigan
Sojin An: Department of Biological Chemistry, University of Michigan
Yang Zhang: Department of Biological Chemistry, University of Michigan
Michael A. Cianfrocco: Department of Biological Chemistry, University of Michigan
Min Su: Life Sciences Institute, University of Michigan
Yali Dou: Department of Biological Chemistry, University of Michigan
Uhn-Soo Cho: Department of Biological Chemistry, University of Michigan

DOI: https://doi.org/10.1038/s41467-019-13550-2
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 13

Abstract

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MLL family histone methyltransferases deposit histone H3 Lys4 mono-/di-/tri-methylation and regulate gene expression in mammals. Here the authors report the single-particle cryo-EM structure of the NCP-bound human MLL1 core complex, shedding light on how the MLL1 complex engages chromatin and how chromatin binding promotes MLL1 tri-methylation activity.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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