Zdorovʹe Rebenka (Feb 2016)

Association of Gene Variations POMP, FLG, MTOR and ATG5 gene with the Risk of Asthma in Children

  • O.P. Volosovets,
  • S.P. Kryvopustov,
  • O.V. Pavlyk,
  • O.V. Yemets,
  • D.O. Stroi,
  • V.Ye. Dosenko

DOI
https://doi.org/10.22141/2224-0551.1.69.2016.73693
Journal volume & issue
Vol. 11, no. 1.69
pp. 18 – 24

Abstract

Read online

The aim of this study was to determine the correlation between single-nucleotide polymorphisms in the proteasome maturation protein gene (POMP), filaggrin gene (FLG), mammalian target of rapamycin gene (MTOR) and autophagy gene (ATG5) and the development of asthma in children. Methods: clinical, genetic — determining the genotype of patients and healthy children using real-time polymerase chain reaction; statistical ones. Results. It was found that the distribution of genotypes in the studied groups was significantly different when analysing polymorphism in POMP gene: GG variant was detected in 9 healthy children and was absent in ill people, 32 patients and 37 healthy children had AG variant, 62 and 52 — allelic AA variant, while minor variant wasn’t detected in patients with asthma at all (p < 0.05 by the χ2 criterion). Variants with the minor genotype of the FLG were found to be 2.5 times more frequent among patients than in control group: 5 patients and 2 healthy children had allelic variant AA, 27 and 36 — heterozygous variant GA, 67 and 61 — GG. Variants with minor genotype in MTOR gene were 2.4 times more often in the control group than in patients: 50 patients and 43 healthy children had major allelic variant TT, 36 patients and 32 healthy children had TC variant, 5 and 12 — CC variant. There was found a statistically significant difference in the distribution of genotypes of polymorphism in ATG5 gene: 51 patients and 43 healthy children were heterozygous (CT), 29 and 21 — had minor variant TT, 18 and 33 — major variant CC (p < 0.05 by the χ2). Minor polymorphism of ATG5 gene was associated with increased risk of early manifestation of the asthma (up to 3 years of life) (p < 0.05 by χ2). MDR-analysis has showed that polymorphism in ATG5 gene was the strongest predictor of asthma as it reduced entropy level by 7.88 %. Polymorphism in the POMP gene reduces entropy level by 5.21 %, polymorphism in the MTOR gene — by 1.18 %, polymorphism in the FLG gene — by 1.04 %. Conclusions. These polymorphisms may serve as prognostic markers for the development of asthma in children.

Keywords