Itaconate and citrate releasing polymer attenuates foreign body response in biofabricated cardiac patches

Dawn Bannerman; Simon Pascual-Gil; Scott Campbell; Richard Jiang; Qinghua Wu; Sargol Okhovatian; Karl T. Wagner; Miles Montgomery; Michael A. Laflamme; Locke Davenport Huyer; Milica Radisic

Materials Today Bio (Feb 2024)

Itaconate and citrate releasing polymer attenuates foreign body response in biofabricated cardiac patches

Dawn Bannerman,
Simon Pascual-Gil,
Scott Campbell,
Richard Jiang,
Qinghua Wu,
Sargol Okhovatian,
Karl T. Wagner,
Miles Montgomery,
Michael A. Laflamme,
Locke Davenport Huyer,
Milica Radisic

Affiliations

Dawn Bannerman: Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, ON, Canada; Institute of Biomedical Engineering, University of Toronto, Toronto, ON, Canada; Toronto General Health Research Institute, University Health Network, Toronto, ON, Canada
Simon Pascual-Gil: Institute of Biomedical Engineering, University of Toronto, Toronto, ON, Canada; Toronto General Health Research Institute, University Health Network, Toronto, ON, Canada
Scott Campbell: Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, ON, Canada; Toronto General Health Research Institute, University Health Network, Toronto, ON, Canada
Richard Jiang: Institute of Biomedical Engineering, University of Toronto, Toronto, ON, Canada; Toronto General Health Research Institute, University Health Network, Toronto, ON, Canada
Qinghua Wu: Institute of Biomedical Engineering, University of Toronto, Toronto, ON, Canada; Toronto General Health Research Institute, University Health Network, Toronto, ON, Canada
Sargol Okhovatian: Institute of Biomedical Engineering, University of Toronto, Toronto, ON, Canada; Toronto General Health Research Institute, University Health Network, Toronto, ON, Canada
Karl T. Wagner: Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, ON, Canada; Toronto General Health Research Institute, University Health Network, Toronto, ON, Canada
Miles Montgomery: Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, ON, Canada; Institute of Biomedical Engineering, University of Toronto, Toronto, ON, Canada; Toronto General Health Research Institute, University Health Network, Toronto, ON, Canada
Michael A. Laflamme: McEwen Stem Cell Institute, University Health Network, Toronto, ON, Canada; Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON, Canada
Locke Davenport Huyer: Applied Oral Sciences, Dalhousie University, Halifax, NS, Canada; School of Biomedical Engineering, Dalhousie University, Halifax, NS, Canada; Department of Microbiology & Immunology, Dalhousie University, Halifax, NS, Canada; Nova Scotia Health, Halifax, NS, Canada
Milica Radisic: Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, ON, Canada; Institute of Biomedical Engineering, University of Toronto, Toronto, ON, Canada; Toronto General Health Research Institute, University Health Network, Toronto, ON, Canada; Corresponding author. Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, ON, Canada.

Journal volume & issue: Vol. 24
p. 100917

Abstract

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Application of cardiac patches to the heart surface can be undertaken to provide support and facilitate regeneration of the damaged cardiac tissue following ischemic injury. Biomaterial composition is an important consideration in the design of cardiac patch materials as it governs host response to ultimately prevent the undesirable fibrotic response. Here, we investigate a novel patch material, poly (itaconate-co-citrate-co-octanediol) (PICO), in the context of cardiac implantation. Citric acid (CA) and itaconic acid (ITA), the molecular components of PICO, provided a level of protection for cardiac cells during ischemic reperfusion injury in vitro. Biofabricated PICO patches were shown to degrade in accelerated and hydrolytic conditions, with CA and ITA being released upon degradation. Furthermore, the host response to PICO patches after implantation on rat epicardium in vivo was explored and compared to two biocompatible cardiac patch materials, poly (octamethylene (anhydride) citrate) (POMaC) and poly (ethylene glycol) diacrylate (PEGDA). PICO patches resulted in less macrophage infiltration and lower foreign body giant cell reaction compared to the other materials, with corresponding reduction in smooth muscle actin-positive vessel infiltration into the implant region. Overall, this work demonstrates that PICO patches release CA and ITA upon degradation, both of which demonstrate cardioprotective effects on cardiac cells after ischemic injury, and that PICO patches generate a reduced inflammatory response upon implantation to the heart compared to other materials, signifying promise for use in cardiac patch applications.

Published in Materials Today Bio

ISSN: 2590-0064 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Medicine: Medicine (General); Science: Biology (General)
Website: https://www.journals.elsevier.com/materials-today-bio

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