Influence of incubation time of Trichoderma harzianum Rifai and antifungal activity of culture filtrate against Bipolaris oryzae

Abstract

This work was developed at Agriculture Microbiology laboratory in the Agriculture Faculty at Universidad Central “Martha Abreu” de Las Villas, with the aim to evaluate the influence of incubation time of Trichoderma harzianum Rifai on antifungal activity of its culture filtrates against Bipolaris oryzae. Flask with Czapek broth was used for growing T. harzianum, then one disc of mycelium were incubated during 20 and 30 days at 28±1ºC and dark. Trichoderma harzianum (strain A-34) was submited to filtration process, and comparison were done among concentration of 0%, 25%, 50%, 75%, 100% (v/v). Major inhibition porcentaje were observed in concentrations of 75% y 100% both at 20 and 30 days of incubation. At 20 day the inhibition porcentaje was greater in comparsison with 30 days.

Keywords

• Bipolaris oryzae
• filtrate of cultures
• inhibition of the mycelial growth
• Trichoderma harzianum