Protocol for generation and engineering of thyroid cell lineages using CRISPR-Cas9 editing to recapitulate thyroid cancer histotype progression

Vincenzo Davide Pantina; Francesco Verona; Alice Turdo; Veronica Veschi; Chiara Modica; Melania Lo Iacono; Miriam Gaggianesi; Sebastiano Di Bella; Matilde Todaro; Simone Di Franco; Giorgio Stassi

STAR Protocols (Sep 2024)

Protocol for generation and engineering of thyroid cell lineages using CRISPR-Cas9 editing to recapitulate thyroid cancer histotype progression

Vincenzo Davide Pantina,
Francesco Verona,
Alice Turdo,
Veronica Veschi,
Chiara Modica,
Melania Lo Iacono,
Miriam Gaggianesi,
Sebastiano Di Bella,
Matilde Todaro,
Simone Di Franco,
Giorgio Stassi

Affiliations

Vincenzo Davide Pantina: Department of Precision Medicine in Medical, Surgical and Critical Care, University of Palermo, 90100 Palermo, Italy
Francesco Verona: Department of Precision Medicine in Medical, Surgical and Critical Care, University of Palermo, 90100 Palermo, Italy
Alice Turdo: Department of Health Promotion, Mother and Child Care, Internal Medicine and Medical Specialties, University of Palermo, 90100 Palermo, Italy
Veronica Veschi: Department of Precision Medicine in Medical, Surgical and Critical Care, University of Palermo, 90100 Palermo, Italy; Department of Molecular Medicine, University La Sapienza, 00161 Rome, Italy
Chiara Modica: Department of Precision Medicine in Medical, Surgical and Critical Care, University of Palermo, 90100 Palermo, Italy
Melania Lo Iacono: Department of Health Promotion, Mother and Child Care, Internal Medicine and Medical Specialties, University of Palermo, 90100 Palermo, Italy
Miriam Gaggianesi: Department of Precision Medicine in Medical, Surgical and Critical Care, University of Palermo, 90100 Palermo, Italy
Sebastiano Di Bella: Department of Precision Medicine in Medical, Surgical and Critical Care, University of Palermo, 90100 Palermo, Italy
Matilde Todaro: Department of Health Promotion, Mother and Child Care, Internal Medicine and Medical Specialties, University of Palermo, 90100 Palermo, Italy; A.O.U.P. “Paolo Giaccone”, University of Palermo, 90100 Palermo, Italy
Simone Di Franco: Department of Precision Medicine in Medical, Surgical and Critical Care, University of Palermo, 90100 Palermo, Italy; Corresponding author
Giorgio Stassi: Department of Precision Medicine in Medical, Surgical and Critical Care, University of Palermo, 90100 Palermo, Italy; Corresponding author

Journal volume & issue: Vol. 5, no. 3
p. 103263

Abstract

Read online

Summary: Thyroid carcinoma represents the first malignancy among the endocrine organs. Investigating the cellular hierarchy and the mechanisms underlying the initiation of thyroid carcinoma is crucial in thyroid cancer research. Here, we present a protocol for deriving thyroid cell lineage from human embryonic stem cells. We also describe steps for engineering thyroid progenitor cells utilizing CRISPR-Cas9 technology, which can be used to perform in vivo studies, thus facilitating the development of representative thyroid tumorigenesis models.For complete details on the use and execution of this protocol, please refer to Veschi et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Published in STAR Protocols

ISSN: 2666-1667 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Science (General)
Website: https://star-protocols.cell.com/

About the journal

Abstract

Keywords