Український стоматологічний альманах (Mar 2015)
COMPARATIVE CHARACTERISTICS OF TRIGLYCERIDE CONTENT IN FEMALE RATS’ PERIODONTAL TISSUES UNDER IMBALANCED RATION
Abstract
This paper presents the survey findings concerning the primary indices of lipid metabolism, i.e. triglycerides in the periodontal tissues of female rats, which were kept on imbalanced rations. Purpose. To compare the triglyceride level in the periodontal tissues of experimental animals, which were kept on imbalanced high carbohydrate and animal-vegetable fat rations. Materials and methods. There were enrolled 40 female Wistar rats, aged 4 months, mean weight 159.95±0.24, in the 7-month trial. Depending on the feeding rations they were distributed into 4 groups (10 animals per each). The 1st group animals were kept on standard diet; the 2nd group animals were kept on easily digested carbohydrates diet (up to 25 per cent); the 3rd group animals were kept on vegetable fat diet (up to 35 per cent); the 4th group animals were kept on animal fat diet (up to 35 per cent). The triglyceride level was determined in the alveolar bone and gum tissue. Results and discussion. The 1st group animals' triglyceride level determined in the alveolar bone and gum tissue was taken as a conventional physiological standard (2.93±0.81 mM/g for alveolar bone, 4.43±0.96 mM/g for gum tissue). The 2nd group animals were found to have the increased index in the alveolar bone (5.71±0.12 mM/g) and in the gum tissue (10.30±0.94 mM/g) (Р2.1≤0.01–0.05). The mentioned above indicated the insulin resistance of the studied tissues as a component of metabolic syndrome. The increase in the triglyceride level in the 3rd group animals was detected to be significantly higher than that in the 2nd one. The triglyceride level in the alveolar bone was observed to be by 1.5 times more than normal, and in the gum tissue it was by 1.3 times more than normal (Р3.1≤0.01–0.05). Adding superfluous vegetable fat to standard food is proved to result in much the largest increase in the level of triglycerides than that when consuming superfluous easily digested carbohydrates. The significant difference between the indices of triglyceride level in the alveolar bone (1.62±0.91 mM/g) and the gum tissue (3.47±0.35 mM/g) in the 4th group animals was absent (Р4.1≥0.01–0.05) as compared to the corresponding indices in the 1st group animals. Conclusions. The level of triglycerides in the periodontal tissues of the experimental animals depends on the food ration. The unbalanced easily digested carbohydrates ration causes the increased triglyceride level in the alveolar bone and the gum tissue, as the sign of insulin resistance. When keeping animals on unbalanced vegetable fat ration the signs of insulin resistance in the periodontal tissues are detected to be increased. The unbalanced animal fat ration does not affect the studied tissues.
