Nature Communications (Oct 2018)
A FRET biosensor for necroptosis uncovers two different modes of the release of DAMPs
- Shin Murai,
- Yoshifumi Yamaguchi,
- Yoshitaka Shirasaki,
- Mai Yamagishi,
- Ryodai Shindo,
- Joanne M. Hildebrand,
- Ryosuke Miura,
- Osamu Nakabayashi,
- Mamoru Totsuka,
- Taichiro Tomida,
- Satomi Adachi-Akahane,
- Sotaro Uemura,
- John Silke,
- Hideo Yagita,
- Masayuki Miura,
- Hiroyasu Nakano
Affiliations
- Shin Murai
- Department of Biochemistry, Toho University School of Medicine
- Yoshifumi Yamaguchi
- Hibernation Metabolism, Physiology, and Development Group, Environmental Biology Division, Institute of Low Temperature Science, Hokkaido University
- Yoshitaka Shirasaki
- Precursory Research for Embryonic Science and Technology, Japan Science and Technology Agency
- Mai Yamagishi
- Department of Biological Sciences, Graduate School of Science, The University of Tokyo
- Ryodai Shindo
- Department of Biochemistry, Toho University School of Medicine
- Joanne M. Hildebrand
- Division of Cell Signaling and Cell Death, The Walter and Eliza Hall Institute of Medical Research
- Ryosuke Miura
- Department of Biochemistry, Toho University School of Medicine
- Osamu Nakabayashi
- Department of Biochemistry, Toho University School of Medicine
- Mamoru Totsuka
- Department of Food Science and Technology, Faculty of Applied Life Science, Nippon Veterinary and Life Science University
- Taichiro Tomida
- Department of Physiology, Toho University School of Medicine
- Satomi Adachi-Akahane
- Department of Physiology, Toho University School of Medicine
- Sotaro Uemura
- Department of Biological Sciences, Graduate School of Science, The University of Tokyo
- John Silke
- Division of Cell Signaling and Cell Death, The Walter and Eliza Hall Institute of Medical Research
- Hideo Yagita
- Department of Immunology, Juntendo University Graduate School of Medicine
- Masayuki Miura
- Department of Genetics, Graduate School of Pharmaceutical Sciences, The University of Tokyo
- Hiroyasu Nakano
- Department of Biochemistry, Toho University School of Medicine
- DOI
- https://doi.org/10.1038/s41467-018-06985-6
- Journal volume & issue
-
Vol. 9,
no. 1
pp. 1 – 17
Abstract
Necroptotic cells activate MLKL and release inflammatory DAMPs, although the underlying regulatory mechanisms of this process are poorly understood. Here, Murai et al. develop a necroptosis-specific FRET sensor (SMART) that monitors MLKL membrane translocation to identify two modes of DAMP release.
