EFFECTS OF 14 DAYS MELATONIN INTRODUCTION ON THE HYDROGEN SULFIDE PRODUCTION IN THE LIVER OF ALLOXAN-INDUCED DIABETES MELLITUS

Abstract

Recently, with the perception of hydrogen sulfide as a gasotransmitter, it came to the understanding that endogenous H2 S is an important component of many biochemical processes in organism. Diabetes mellitus (DM) is a disease that is expanding globally and is considered an epidemic. DM has many complications, including pathological changes in the liver, associated with oxidative stress. However, the role of hydrogen sulfide in diabetes mellitus is insufficiently studied. Melatonin is a potent, naturally occurring antioxidant and a signaling molecule based on its primary and secondarily-evolved functions in organisms. Thus, this study has the following purpose: to examine the role of melatonin introduction on the hydrogen sulfide production in the liver of alloxan induced diabetic rats. Experiments were conducted on white outbred sexually mature male rats with the body weight – 0,15-0,18 kg. Diabetes was induced by a single intraperitoneal injection of freshly prepared alloxan monohydrate (150 mg/kg body weight). The rats were divided into the following groups: controls rats; diabetes; diabetes + melatonin (animals with diabetes were introduced the melatonin (Merck, Germany) intragastrically in the dose of 10 mg/kg at 8 a.m. daily during 14 days). In the liver was determined content of H2 S concentration, H2 S production and activities of CSE, CBS and CAT. It was found that alloxan diabetes was observed an decrease H2 S concentration and an increase H2 S production in the liver compared to control group. The activities of CSE, CBS and CAT in alloxan diabetic rats was higher compared to controls rats. Introduction of the melatonin increased H2 S concentration and decreased content of H2 S production compared to diabetic group. There was also a decrease the activities of CSE, CBS and CAT in the liver compared to the alloxan diabetic rats. We have also found the positive correlations between investigated parameters, which indicate connection between concentration and production of H2 S and the enzyme activities of its synthesis, and the relationship between these enzymes. The administration of melatonin normalizes concentration of H2 S and CSE activity in the liver of alloxan diabetic rats, which contributes to lowering glucose levels in the blood, but the reduction of H2 S production, CBS and CAT activities did not have level of control group. The positive effect of melatonin may be due to increased antioxidant protection of organism. Further research will be study the mechanisms of melatonin effects on the exchange indicators of hydrogen sulfide at alloxane-induced diabetes.

Keywords

• diabetes mellitus
• rats
• melatonin
• liver
• hydrogen sulfide.