Identification and Molecular Characterization of Telosma Mosaic Virus (TelMV) and East Asian Passiflora Virus (EAPV) from Patchouli in China
Asma Aziz,
Na Li,
Xiaoqing Wang,
Linxi Wang,
Yougen Wu,
Muhammad Zeeshan Ul Haq,
Zhaoji Dai,
Hongguang Cui
Affiliations
Asma Aziz
Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests (Ministry of Education), School of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China
Na Li
Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests (Ministry of Education), School of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China
Xiaoqing Wang
Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests (Ministry of Education), School of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China
Linxi Wang
Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests (Ministry of Education), School of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China
Yougen Wu
School of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication), Hainan University, Sanya 572025, China
Muhammad Zeeshan Ul Haq
School of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication), Hainan University, Sanya 572025, China
Zhaoji Dai
Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests (Ministry of Education), School of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China
Hongguang Cui
Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests (Ministry of Education), School of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China
Patchouli is a valuable medicinal herb and cash crop in China, but viral infections cause significant yield losses. This study identified six viruses in patchouli transcriptome data, including the first-ever detection of East Asian Passiflora Virus (EAPV) in patchouli. RT-PCR validated three viruses from diseased patchouli plants in Haikou, China: telosma tosaic virus (TelMV), broad bean wilt virus-2 (BBWV-2), and pogostemom alphacytorhabdovirus 1 (PogACRV1_Pog). The complete genomic sequence of TelMV from patchouli (TelMV) was determined, revealing a 9691-nucleotide RNA genome encoding a 3083-amino-acid polyprotein. Comparative analysis showed 77.66% to 81.01% nucleotide sequence identity with previously reported TelMV isolates. TelMV was also shown to be infectious in Nicotiana benthamiana through sap rub-inoculation. Additionally, a large portion of the EAPV genome was reconstructed from RNA-seq data, with coat protein analysis confirming its identity. This study presents the first complete TelMV genome in patchouli and the first detection of EAPV in the plant.
