Combining Ability Analysis and Marker-Based Prediction of Heterosis in Yield Reveal Prominent Heterotic Combinations from Diallel Population of Rice

Md. Mobinur Rahman; Umakanta Sarker; Md Ahsanul Haque Swapan; Mohammad Sharif Raihan; Shinya Oba; Saud Alamri; Manzer H. Siddiqui

doi:10.3390/agronomy12081797

Agronomy (Jul 2022)

Combining Ability Analysis and Marker-Based Prediction of Heterosis in Yield Reveal Prominent Heterotic Combinations from Diallel Population of Rice

Md. Mobinur Rahman,
Umakanta Sarker,
Md Ahsanul Haque Swapan,
Mohammad Sharif Raihan,
Shinya Oba,
Saud Alamri,
Manzer H. Siddiqui

Affiliations

Md. Mobinur Rahman: Department of Genetics and Plant Breeding, Faculty of Agriculture, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur 1706, Bangladesh
Umakanta Sarker: Department of Genetics and Plant Breeding, Faculty of Agriculture, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur 1706, Bangladesh
Md Ahsanul Haque Swapan: Department of Entomology, Faculty of Agriculture, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur 1706, Bangladesh
Mohammad Sharif Raihan: Department of Genetics and Plant Breeding, Faculty of Agriculture, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur 1706, Bangladesh
Shinya Oba: Laboratory of Field Science, Faculty of Applied Biological Sciences, Gifu University, Yanagido 1-1, Gifu 501-1193, Japan
Saud Alamri: Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia
Manzer H. Siddiqui: Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia

DOI: https://doi.org/10.3390/agronomy12081797
Journal volume & issue: Vol. 12, no. 8
p. 1797

Abstract

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Combining ability along with heterosis was elucidated in the 21 F1 hybrid population of rice derived from a 7 × 7 diallel mating design. Furthermore, to formulate a strategy for marker-based prediction of heterosis, the molecular genetic diversity (GD) was then surveyed among the seven parental lines with RAPD markers. Analysis of combining ability revealed significant GCA and SCA variances and the ratio between the aforesaid genetic parameter was more than unity for most of the traits signifying the preponderance of additive gene action. Hybrid combination Double Rice × Pokkali was the best specific combiner for the traits pollen fertility percentage (%), panicle length (cm), filled grains/panicle (no.) and grain yield/hill (g) while Muktagacha × BRRI dhan29 and Basmati × Double Rice were the best specific combiners for grain length (mm) and 1000-grain weight (g), respectively. Regarding the magnitude of heterosis, Double Rice × Pokkali exhibited maximum heterobeltiosis for panicle length (33.33%) and the number of filled grains/panicle (144.01%). Furthermore, Pokkali × Chinigura was verified for producing the highest heterobeltiosis for tillers/hill (173.17%), as well as grain yield/hill (71.05%). The heatmap relying on molecular genetic diversity exposed the highest genetic distance between Chinigura and Pokkali followed by Muktagacha and Pokkali. Unweighted neighbor-joining trees clustered the parental lines into three groups indicating the presence of considerable genetic diversity among those seven parental genotypes. A positive and significant correlation was explored between molecular GD with specific combining ability as well as heterosis for all of the traits revealing the huge potentiality of hybrid performance prediction using RAPD markers with the present set of individuals. Thus, exploration of definite genomic regions underlying the candidate genes for respective traits relying on such RAPD-generated data following an advanced molecular marker approach would pave the path of mining out yielder heterotic combinations.

Published in Agronomy

ISSN: 2073-4395 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Agriculture
Website: http://www.mdpi.com/journal/agronomy

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