Evidence for functional pre-coupled complexes of receptor heteromers and adenylyl cyclase

Gemma Navarro; Arnau Cordomí; Verónica Casadó-Anguera; Estefanía Moreno; Ning-Sheng Cai; Antoni Cortés; Enric I. Canela; Carmen W. Dessauer; Vicent Casadó; Leonardo Pardo; Carme Lluís; Sergi Ferré

doi:10.1038/s41467-018-03522-3

Nature Communications (Mar 2018)

Evidence for functional pre-coupled complexes of receptor heteromers and adenylyl cyclase

Gemma Navarro,
Arnau Cordomí,
Verónica Casadó-Anguera,
Estefanía Moreno,
Ning-Sheng Cai,
Antoni Cortés,
Enric I. Canela,
Carmen W. Dessauer,
Vicent Casadó,
Leonardo Pardo,
Carme Lluís,
Sergi Ferré

Affiliations

Gemma Navarro: Department of Biochemistry and Physiology of the Faculty of Pharmacy of the University of Barcelona
Arnau Cordomí: Laboratory of Computational Medicine, School of Medicine, Autonomous University of Barcelona
Verónica Casadó-Anguera: Department of Biochemistry and Molecular Biomedicine of the Faculty of Biology and Institute of Biomedicine of the University of Barcelona and Center for Biomedical Research in Neurodegenerative Diseases Network
Estefanía Moreno: Department of Biochemistry and Molecular Biomedicine of the Faculty of Biology and Institute of Biomedicine of the University of Barcelona and Center for Biomedical Research in Neurodegenerative Diseases Network
Ning-Sheng Cai: Integrative Neurobiology Section, National Institute on Drug Abuse, National Institutes of Health
Antoni Cortés: Department of Biochemistry and Molecular Biomedicine of the Faculty of Biology and Institute of Biomedicine of the University of Barcelona and Center for Biomedical Research in Neurodegenerative Diseases Network
Enric I. Canela: Department of Biochemistry and Molecular Biomedicine of the Faculty of Biology and Institute of Biomedicine of the University of Barcelona and Center for Biomedical Research in Neurodegenerative Diseases Network
Carmen W. Dessauer: Department of Integrative Biology and Pharmacology, McGovern Medical School, University of Texas Health Science Center
Vicent Casadó: Department of Biochemistry and Molecular Biomedicine of the Faculty of Biology and Institute of Biomedicine of the University of Barcelona and Center for Biomedical Research in Neurodegenerative Diseases Network
Leonardo Pardo: Laboratory of Computational Medicine, School of Medicine, Autonomous University of Barcelona
Carme Lluís: Department of Biochemistry and Molecular Biomedicine of the Faculty of Biology and Institute of Biomedicine of the University of Barcelona and Center for Biomedical Research in Neurodegenerative Diseases Network
Sergi Ferré: Integrative Neurobiology Section, National Institute on Drug Abuse, National Institutes of Health

DOI: https://doi.org/10.1038/s41467-018-03522-3
Journal volume & issue: Vol. 9, no. 1
pp. 1 – 12

Abstract

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It is unclear whether GPCRs, G proteins and adenylyl cyclase (AC) associate through random collisions or defined pre-coupling mechanisms. Using a peptide-based approach, the authors show that heteromers of adenosine A2A and dopamine D2 receptors form pre-coupled complexes with their cognate G proteins and AC5.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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