Nature Communications (Jul 2021)
High-resolution characterization of gene function using single-cell CRISPR tiling screen
- Lu Yang,
- Anthony K. N. Chan,
- Kazuya Miyashita,
- Christopher D. Delaney,
- Xi Wang,
- Hongzhi Li,
- Sheela Pangeni Pokharel,
- Sandra Li,
- Mingli Li,
- Xiaobao Xu,
- Wei Lu,
- Qiao Liu,
- Nicole Mattson,
- Kevin Yining Chen,
- Jinhui Wang,
- Yate-Ching Yuan,
- David Horne,
- Steven T. Rosen,
- Yadira Soto-Feliciano,
- Zhaohui Feng,
- Takayuki Hoshii,
- Gang Xiao,
- Markus Müschen,
- Jianjun Chen,
- Scott A. Armstrong,
- Chun-Wei Chen
Affiliations
- Lu Yang
- Department of Systems Biology, Beckman Research Institute
- Anthony K. N. Chan
- Department of Systems Biology, Beckman Research Institute
- Kazuya Miyashita
- Department of Systems Biology, Beckman Research Institute
- Christopher D. Delaney
- Department of Pediatric Oncology, Dana-Farber Cancer Institute—Harvard Medical School
- Xi Wang
- Department of Pediatric Oncology, Dana-Farber Cancer Institute—Harvard Medical School
- Hongzhi Li
- City of Hope Comprehensive Cancer Center
- Sheela Pangeni Pokharel
- Department of Systems Biology, Beckman Research Institute
- Sandra Li
- Department of Systems Biology, Beckman Research Institute
- Mingli Li
- Department of Systems Biology, Beckman Research Institute
- Xiaobao Xu
- Department of Systems Biology, Beckman Research Institute
- Wei Lu
- Department of Systems Biology, Beckman Research Institute
- Qiao Liu
- Department of Systems Biology, Beckman Research Institute
- Nicole Mattson
- Department of Systems Biology, Beckman Research Institute
- Kevin Yining Chen
- Department of Pediatric Oncology, Dana-Farber Cancer Institute—Harvard Medical School
- Jinhui Wang
- City of Hope Comprehensive Cancer Center
- Yate-Ching Yuan
- City of Hope Comprehensive Cancer Center
- David Horne
- City of Hope Comprehensive Cancer Center
- Steven T. Rosen
- City of Hope Comprehensive Cancer Center
- Yadira Soto-Feliciano
- Rockefeller University
- Zhaohui Feng
- Department of Pediatric Oncology, Dana-Farber Cancer Institute—Harvard Medical School
- Takayuki Hoshii
- Department of Pediatric Oncology, Dana-Farber Cancer Institute—Harvard Medical School
- Gang Xiao
- Department of Systems Biology, Beckman Research Institute
- Markus Müschen
- Department of Systems Biology, Beckman Research Institute
- Jianjun Chen
- Department of Systems Biology, Beckman Research Institute
- Scott A. Armstrong
- Department of Pediatric Oncology, Dana-Farber Cancer Institute—Harvard Medical School
- Chun-Wei Chen
- Department of Systems Biology, Beckman Research Institute
- DOI
- https://doi.org/10.1038/s41467-021-24324-0
- Journal volume & issue
-
Vol. 12,
no. 1
pp. 1 – 9
Abstract
Identifying functional domains and genetic regulatory mechanisms is essential for developing new therapies. Here the authors present sc-Tiling, single-cell high-density CRISPR tiling screening for functional domain characterization.
