Effect of induced gestational diabetes on TBX20 gene expression in the heart of the 11.5-day-old embryo of the C57BL/6 mouse

Abstract

Background and Objective: Diabetes mellitus is one of the most common metabolic and global health threats. The gene needed for the development of the TBX20 gene is the fusion of the gene, and the defect in the sequence and expression of this gene also causes heart defects. Due to significant prevalence of gestational diabetes mellitus in human population, this study was done to evaluate the effect of TBX20-induced gestational diabetes mellitus in the heart of the mice embryo at 11.5 days. Methods: This experimental study was done on induced diabetic C57BL/6 female mice. Gestational diabetes induced by interaperitoneal injection of sterptozotocin at GD1. On the day of pregnancy 11.5, embryonic heart samples from these mice were isolated. After extraction of RNA, cDNA synthesis of RNA was performed. The Real Time-PCR technique was used to determine the expression of TBX20 gene. Expression level of TBX20 gene in experimental and control was calculated using the 2–∆∆CT method. Results: Expression of TBX20 gene in diabetic specimens was twice as high as the control samples, which was statistically significant (P<0.05). Conclusion: It seems, increasing TBX20 gene expression in embryonic heart tissue may be one of the complications of gestational diabetes mellitus and can lead to abnormalities in the heart embryo.

Keywords

• gestational diabetes
• tbx20 gene
• heart
• embryonic development