Química Nova (Jan 2012)

High performance thin layer chromatographic determination of nifedipine in human serum after liquid-liquid extraction

  • Sigrid Mennickent,
  • Jonathan Contreras,
  • Berta Schulz,
  • Marta de Diego,
  • Mario Vega

DOI
https://doi.org/10.1590/S0100-40422012000200032
Journal volume & issue
Vol. 35, no. 2
pp. 411 – 415

Abstract

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A method using HPTLC for quantitation of nifedipine in serum was developed and validated. It includes a liquid-liquid extraction, and carbamazepine as internal standard. Chloroform: ethyl acetate: cyclohexane (19:2:2, v/v/v) was the mobile phase. The method showed good relationship (r = 0.996) (2.00 to 25.00 ng/band, corresponding to 0.02 and 0.25 ng/µL in serum). The % RSD of intra-assay and inter-assay, were between 0.57 and 3.56 and 1.16 to 3.60, respectively. LOD and LOQ were 0.72 and 0.86 ng/band, respectively. The recovery values were between 93 and 102%. Rf for nifedipine and carbamazepine were 0.31 and 0.10, respectively.

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