Frontiers in Public Health (Sep 2022)
Molecular prevalence and genetic diversity analysis of Enterocytozoon bieneusi in humans in Hainan Province, China: High diversity and unique endemic genetic characteristics
- Tiemin Zhang,
- Guangxu Ren,
- Guangxu Ren,
- Guangxu Ren,
- Huanhuan Zhou,
- Huanhuan Zhou,
- Huanhuan Zhou,
- Yu Qiang,
- Yu Qiang,
- Yu Qiang,
- Jiaqi Li,
- Jiaqi Li,
- Jiaqi Li,
- Yun Zhang,
- Yun Zhang,
- Yun Zhang,
- Tingting Li,
- Tingting Li,
- Tingting Li,
- Yunfei Zhou,
- Yunfei Zhou,
- Yunfei Zhou,
- Yuan Wang,
- Yuan Wang,
- Yuan Wang,
- Xiuyi Lai,
- Xiuyi Lai,
- Xiuyi Lai,
- Shen Lei,
- Shen Lei,
- Shen Lei,
- Feng Tan,
- Rui Liu,
- Wenting Li,
- Jing He,
- Wei Zhao,
- Chuanlong Zhu,
- Gang Lu,
- Gang Lu,
- Gang Lu,
- Gang Lu
Affiliations
- Tiemin Zhang
- Department of General Surgery, The Second Affiliated Hospital of Hainan Medical University, Haikou, China
- Guangxu Ren
- Key Laboratory of Tropical Translational Medicine of Ministry of Education, Hainan Medical University, Haikou, China
- Guangxu Ren
- Hainan Medical University-The University of Hong Kong Joint Laboratory of Tropical Infectious Diseases, Hainan Medical University, Haikou, China
- Guangxu Ren
- Department of Pathogenic Biology, Hainan Medical University, Haikou, China
- Huanhuan Zhou
- Key Laboratory of Tropical Translational Medicine of Ministry of Education, Hainan Medical University, Haikou, China
- Huanhuan Zhou
- Hainan Medical University-The University of Hong Kong Joint Laboratory of Tropical Infectious Diseases, Hainan Medical University, Haikou, China
- Huanhuan Zhou
- Department of Pathogenic Biology, Hainan Medical University, Haikou, China
- Yu Qiang
- Key Laboratory of Tropical Translational Medicine of Ministry of Education, Hainan Medical University, Haikou, China
- Yu Qiang
- Hainan Medical University-The University of Hong Kong Joint Laboratory of Tropical Infectious Diseases, Hainan Medical University, Haikou, China
- Yu Qiang
- Department of Pathogenic Biology, Hainan Medical University, Haikou, China
- Jiaqi Li
- Key Laboratory of Tropical Translational Medicine of Ministry of Education, Hainan Medical University, Haikou, China
- Jiaqi Li
- Hainan Medical University-The University of Hong Kong Joint Laboratory of Tropical Infectious Diseases, Hainan Medical University, Haikou, China
- Jiaqi Li
- Department of Pathogenic Biology, Hainan Medical University, Haikou, China
- Yun Zhang
- Key Laboratory of Tropical Translational Medicine of Ministry of Education, Hainan Medical University, Haikou, China
- Yun Zhang
- Hainan Medical University-The University of Hong Kong Joint Laboratory of Tropical Infectious Diseases, Hainan Medical University, Haikou, China
- Yun Zhang
- Department of Pathogenic Biology, Hainan Medical University, Haikou, China
- Tingting Li
- Key Laboratory of Tropical Translational Medicine of Ministry of Education, Hainan Medical University, Haikou, China
- Tingting Li
- Hainan Medical University-The University of Hong Kong Joint Laboratory of Tropical Infectious Diseases, Hainan Medical University, Haikou, China
- Tingting Li
- Department of Pathogenic Biology, Hainan Medical University, Haikou, China
- Yunfei Zhou
- Key Laboratory of Tropical Translational Medicine of Ministry of Education, Hainan Medical University, Haikou, China
- Yunfei Zhou
- Hainan Medical University-The University of Hong Kong Joint Laboratory of Tropical Infectious Diseases, Hainan Medical University, Haikou, China
- Yunfei Zhou
- Department of Pathogenic Biology, Hainan Medical University, Haikou, China
- Yuan Wang
- Key Laboratory of Tropical Translational Medicine of Ministry of Education, Hainan Medical University, Haikou, China
- Yuan Wang
- Hainan Medical University-The University of Hong Kong Joint Laboratory of Tropical Infectious Diseases, Hainan Medical University, Haikou, China
- Yuan Wang
- Department of Pathogenic Biology, Hainan Medical University, Haikou, China
- Xiuyi Lai
- Key Laboratory of Tropical Translational Medicine of Ministry of Education, Hainan Medical University, Haikou, China
- Xiuyi Lai
- Hainan Medical University-The University of Hong Kong Joint Laboratory of Tropical Infectious Diseases, Hainan Medical University, Haikou, China
- Xiuyi Lai
- Department of Pathogenic Biology, Hainan Medical University, Haikou, China
- Shen Lei
- Key Laboratory of Tropical Translational Medicine of Ministry of Education, Hainan Medical University, Haikou, China
- Shen Lei
- Hainan Medical University-The University of Hong Kong Joint Laboratory of Tropical Infectious Diseases, Hainan Medical University, Haikou, China
- Shen Lei
- Department of Pathogenic Biology, Hainan Medical University, Haikou, China
- Feng Tan
- Department of Parasitology, Wenzhou Medical University, Wenzhou, China
- Rui Liu
- Department of Tropical Diseases, The Second Affiliated Hospital of Hainan Medical University, Haikou, China
- Wenting Li
- Department of Tropical Diseases, The Second Affiliated Hospital of Hainan Medical University, Haikou, China
- Jing He
- Department of Tropical Diseases, The Second Affiliated Hospital of Hainan Medical University, Haikou, China
- Wei Zhao
- Department of Parasitology, Wenzhou Medical University, Wenzhou, China
- Chuanlong Zhu
- Department of Tropical Diseases, The Second Affiliated Hospital of Hainan Medical University, Haikou, China
- Gang Lu
- Key Laboratory of Tropical Translational Medicine of Ministry of Education, Hainan Medical University, Haikou, China
- Gang Lu
- Hainan Medical University-The University of Hong Kong Joint Laboratory of Tropical Infectious Diseases, Hainan Medical University, Haikou, China
- Gang Lu
- Department of Pathogenic Biology, Hainan Medical University, Haikou, China
- Gang Lu
- Department of Tropical Diseases, The Second Affiliated Hospital of Hainan Medical University, Haikou, China
- DOI
- https://doi.org/10.3389/fpubh.2022.1007130
- Journal volume & issue
-
Vol. 10
Abstract
Enterocytozoon bieneusi is a zoonotic pathogen commonly found in humans and animals all over the world. Here, we investigated the occurrence and genotype constitute of E. bieneusi among the individuals from Haikou city of Hainan, China. A total of 1,264 fecal samples of humans were collected, including 628 samples from patients with diarrhea (325 adults and 303 children) and 636 samples from the asymptomatic population (383 college students and 253 kindergarten children). E. bieneusi was detected using nested polymerase chain reaction (PCR) amplification of the internal transcribed spacer (ITS) region. A phylogenetic tree was constructed using a neighbor-joining tree construction method. The overall prevalence of E. bieneusi was 3.7% (47/1,264), while it was 5.6% in the patients with diarrhea (5.8% in adults and 5.3% in children) and 1.9% in the asymptomatic population (2.9% in college students and 0.4% in kindergarten children). The prevalence of E. bieneusi in humans with diarrhea was significantly higher than that in the asymptomatic population (χ2 = 36.9; P < 0.05). A total of 28 genotypes were identified, including ten known genotypes: CHG2 (n = 3), CHG3 (n = 5), CHG5 (n = 10), CM21 (n = 1), EbpA (n = 1), EbpC (n = 1), PigEBITS4 (n = 1), PigEBITS7 (n = 1), SHR1 (n = 4), Type IV (n = 2), and 18 novel genotypes (HNH-1 to HNH-18; one each). All these genotypes were categorized into three groups, including group 1 (n = 6), group 2 (n = 14), and group 13 (n = 8). This was the first study on the identification of E. bieneusi among humans in Hainan, China. The correlation between E. bieneusi infection and diarrhea was observed. The high diversity and distinctive distribution of E. bieneusi genotypes found in this study reflected the unique epidemic genetic characteristics of E. bieneusi in humans living in Hainan.
Keywords
