Paper-Based Screen-Printed Ionic-Liquid/Graphene Electrode Integrated with Prussian Blue/MXene Nanocomposites Enabled Electrochemical Detection for Glucose Sensing
Wisanu Niamsi,
Nutcha Larpant,
Pramod K. Kalambate,
Vitsarut Primpray,
Chanpen Karuwan,
Nadnudda Rodthongkum,
Wanida Laiwattanapaisal
Affiliations
Wisanu Niamsi
Graduate Program in Clinical Biochemistry and Molecular Medicine, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok 10330, Thailand
Nutcha Larpant
Biosensors and Bioanalytical Technology for Cells and Innovative Testing Device Research Unit, Department of Clinical Chemistry, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok 10330, Thailand
Pramod K. Kalambate
Biosensors and Bioanalytical Technology for Cells and Innovative Testing Device Research Unit, Department of Clinical Chemistry, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok 10330, Thailand
Vitsarut Primpray
Graphene Sensor Laboratory (GPL), Graphene and Printed Electronics for Dual-Use Applications Research Division (GPERD), National Security and Dual-Use Technology Center (NSD), National Science and Technology Development Agency (NSTDA), Phahonyothin Road, Khlong Nueng, Khlong Luang, Pathum Thani 12120, Thailand
Chanpen Karuwan
Graphene Sensor Laboratory (GPL), Graphene and Printed Electronics for Dual-Use Applications Research Division (GPERD), National Security and Dual-Use Technology Center (NSD), National Science and Technology Development Agency (NSTDA), Phahonyothin Road, Khlong Nueng, Khlong Luang, Pathum Thani 12120, Thailand
Nadnudda Rodthongkum
Metallurgy and Materials Science Research Institute, Chulalongkorn University, Bangkok 10330, Thailand
Wanida Laiwattanapaisal
Biosensors and Bioanalytical Technology for Cells and Innovative Testing Device Research Unit, Department of Clinical Chemistry, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok 10330, Thailand
As glucose biosensors play an important role in glycemic control, which can prevent the diabetic complications, the development of a glucose sensing platform is still in needed. Herein, the first proposal on the in-house fabricated paper-based screen-printed ionic liquid/graphene electrode (SPIL-GE) modified with MXene (Ti3C2Tx), prussian blue (PB), glucose oxidase (GOx), and Nafion is reported. The concentration of PB/Ti3C2Tx was optimized and the optimal detection potential of PB/Ti3C2Tx/GOx/Nafion/SPIL-GE is −0.05 V. The performance of PB/Ti3C2Tx/GOx/Nafion modified SPIL-GE was characterized by cyclic voltammetry and chronoamperometry technique. This paper-based platform integrated with nanomaterial composites were realized for glucose in the range of 0.0–15.0 mM with the correlation coefficient R2 = 0.9937. The limit of detection method and limit of quantification were 24.5 μM and 81.7 μM, respectively. In the method comparison, this PB/Ti3C2Tx/GOx/Nafion/SPIL-GE exhibits a good correlation with the reference hexokinase method. This novel glucose sensing platform can potentially be used for the good practice to enhance the sensitivity and open the opportunity to develop paper-based electroanalytical devices.
