Analysis on gut microbiota diversity of wild Asian elephants (Elephas maximus) from three regions of Yunnan Province

Abstract

Abstract Studying the gut microbiome diversity of Asian elephants (Elephas maximus) is crucial for understanding their environmental adaptability, health status, and conservation needs. In this study, high-throughput sequencing of the 16S rRNA gene was utilized to analyze and compare the microbial community composition and diversity of 50 wild Asian elephants from three regions in Yunnan Province. The results indicated significant differences in gut microbiome richness among the regions, and the lowest diversity observed in the Lincang region. Principal coordinate analysis (PCoA) revealed that the microbial community structure of the Lincang population was markedly different from that of the other two regions. At the phylum level, Firmicutes, Proteobacteria, and Bacteroidetes were the dominant bacterial groups across all three regions. However, in the Lincang region, the abundance of Proteobacteria was the highest and significantly greater than in the other regions. Additionally, the levels of potential pathogenic bacteria, such as Acinetobacter and Stenotrophomonas, were significantly elevated in the Lincang population compared to the other two regions. Therefore, future conservation efforts need to integrate ecological restoration with microbiome monitoring to mitigate the microbial dysbiosis caused by human disturbances.

Keywords

• Asian elephant (Elephas maximus)
• Gut microbiome diversity and richness
• Gut microbiome function
• Wild Asian elephant management